Compartmental localization of gamma-aminobutyric acid type B receptors in the cholinergic circuitry of the rabbit retina
Zucker, C L ; Nilson, J E ; Ehinger, Berndt LU
and Grzywacz, N M
(2005)
In Journal of Comparative Neurology
493(3).
p.448-459
- Abstract
- Although many effects of gamma-aminobutyric acid (GABA) on retinal function have been attributed to GABA(A) and GABA(C) receptors, specific retinal functions have also been shown to be mediated by GABA(B) receptors, including facilitation of light-evoked acetylcholine release from the rabbit retina (Neal and Cunningham [1995] J. Physiol. 482:363-372). To explain the results of a rich set of experiments, Neal and Cunningham proposed a model for this facilitation. In this model, GABA(B) receptor-mediated inhibition of glycinergic cells would reduce their own inhibition of cholinergic cells. In turn, muscarinic input from the latter to the glycinergic cells would complete a negative-feedback circuitry. In this study, we have used... (More)
- Although many effects of gamma-aminobutyric acid (GABA) on retinal function have been attributed to GABA(A) and GABA(C) receptors, specific retinal functions have also been shown to be mediated by GABA(B) receptors, including facilitation of light-evoked acetylcholine release from the rabbit retina (Neal and Cunningham [1995] J. Physiol. 482:363-372). To explain the results of a rich set of experiments, Neal and Cunningham proposed a model for this facilitation. In this model, GABA(B) receptor-mediated inhibition of glycinergic cells would reduce their own inhibition of cholinergic cells. In turn, muscarinic input from the latter to the glycinergic cells would complete a negative-feedback circuitry. In this study, we have used immunohistochemical techniques to test elements of this model. We report that glycinergic amacrine cells are GABA(B) receptor negative. In contrast, our data reveal the localization of GABA(B) receptors on cholinergic/GABAergic starburst amacrine cells. High-resolution localization of GABA(B) receptors on starburst amacrine cells shows that they are discretely localized to a limited population of its varicosities, the majority of likely synaptic-release sites being devoid of detectable levels of GABA(B) receptors. Finally, we identify a glycinergic cell that is a potential muscarinic receptor-bearing target of GABA(B)-modulated acetylcholine release. This target is the DAPI-3 cell. We propose, based on these data, a modification of the Neal and Cunningham model in which GABA(B) receptors are on starburst, not glycinergic amacrine cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/212142
- author
- Zucker, C L
; Nilson, J E
; Ehinger, Berndt
LU
and Grzywacz, N M
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- directional selectivity, receptor, muscarinic, ganglion cell, amacrine cell, acetylcholine, glycine, receptor targeting
- in
- Journal of Comparative Neurology
- volume
- 493
- issue
- 3
- pages
- 448 - 459
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:16261535
- wos:000233390400009
- scopus:27944436547
- ISSN
- 1096-9861
- DOI
- 10.1002/cne.20766
- language
- English
- LU publication?
- yes
- id
- 8abd20dd-9ed0-4062-8f8d-f17531af566c (old id 212142)
- date added to LUP
- 2016-04-01 12:30:43
- date last changed
- 2022-01-27 06:05:21
@article{8abd20dd-9ed0-4062-8f8d-f17531af566c,
abstract = {{Although many effects of gamma-aminobutyric acid (GABA) on retinal function have been attributed to GABA(A) and GABA(C) receptors, specific retinal functions have also been shown to be mediated by GABA(B) receptors, including facilitation of light-evoked acetylcholine release from the rabbit retina (Neal and Cunningham [1995] J. Physiol. 482:363-372). To explain the results of a rich set of experiments, Neal and Cunningham proposed a model for this facilitation. In this model, GABA(B) receptor-mediated inhibition of glycinergic cells would reduce their own inhibition of cholinergic cells. In turn, muscarinic input from the latter to the glycinergic cells would complete a negative-feedback circuitry. In this study, we have used immunohistochemical techniques to test elements of this model. We report that glycinergic amacrine cells are GABA(B) receptor negative. In contrast, our data reveal the localization of GABA(B) receptors on cholinergic/GABAergic starburst amacrine cells. High-resolution localization of GABA(B) receptors on starburst amacrine cells shows that they are discretely localized to a limited population of its varicosities, the majority of likely synaptic-release sites being devoid of detectable levels of GABA(B) receptors. Finally, we identify a glycinergic cell that is a potential muscarinic receptor-bearing target of GABA(B)-modulated acetylcholine release. This target is the DAPI-3 cell. We propose, based on these data, a modification of the Neal and Cunningham model in which GABA(B) receptors are on starburst, not glycinergic amacrine cells.}},
author = {{Zucker, C L and Nilson, J E and Ehinger, Berndt and Grzywacz, N M}},
issn = {{1096-9861}},
keywords = {{directional selectivity; receptor; muscarinic; ganglion cell; amacrine cell; acetylcholine; glycine; receptor targeting}},
language = {{eng}},
number = {{3}},
pages = {{448--459}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Comparative Neurology}},
title = {{Compartmental localization of gamma-aminobutyric acid type B receptors in the cholinergic circuitry of the rabbit retina}},
url = {{http://dx.doi.org/10.1002/cne.20766}},
doi = {{10.1002/cne.20766}},
volume = {{493}},
year = {{2005}},
}