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Compartmental localization of gamma-aminobutyric acid type B receptors in the cholinergic circuitry of the rabbit retina

Zucker, C L; Nilson, J E; Ehinger, Berndt LU and Grzywacz, N M (2005) In Journal of Comparative Neurology 493(3). p.448-459
Abstract
Although many effects of gamma-aminobutyric acid (GABA) on retinal function have been attributed to GABA(A) and GABA(C) receptors, specific retinal functions have also been shown to be mediated by GABA(B) receptors, including facilitation of light-evoked acetylcholine release from the rabbit retina (Neal and Cunningham [1995] J. Physiol. 482:363-372). To explain the results of a rich set of experiments, Neal and Cunningham proposed a model for this facilitation. In this model, GABA(B) receptor-mediated inhibition of glycinergic cells would reduce their own inhibition of cholinergic cells. In turn, muscarinic input from the latter to the glycinergic cells would complete a negative-feedback circuitry. In this study, we have used... (More)
Although many effects of gamma-aminobutyric acid (GABA) on retinal function have been attributed to GABA(A) and GABA(C) receptors, specific retinal functions have also been shown to be mediated by GABA(B) receptors, including facilitation of light-evoked acetylcholine release from the rabbit retina (Neal and Cunningham [1995] J. Physiol. 482:363-372). To explain the results of a rich set of experiments, Neal and Cunningham proposed a model for this facilitation. In this model, GABA(B) receptor-mediated inhibition of glycinergic cells would reduce their own inhibition of cholinergic cells. In turn, muscarinic input from the latter to the glycinergic cells would complete a negative-feedback circuitry. In this study, we have used immunohistochemical techniques to test elements of this model. We report that glycinergic amacrine cells are GABA(B) receptor negative. In contrast, our data reveal the localization of GABA(B) receptors on cholinergic/GABAergic starburst amacrine cells. High-resolution localization of GABA(B) receptors on starburst amacrine cells shows that they are discretely localized to a limited population of its varicosities, the majority of likely synaptic-release sites being devoid of detectable levels of GABA(B) receptors. Finally, we identify a glycinergic cell that is a potential muscarinic receptor-bearing target of GABA(B)-modulated acetylcholine release. This target is the DAPI-3 cell. We propose, based on these data, a modification of the Neal and Cunningham model in which GABA(B) receptors are on starburst, not glycinergic amacrine cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
directional selectivity, receptor, muscarinic, ganglion cell, amacrine cell, acetylcholine, glycine, receptor targeting
in
Journal of Comparative Neurology
volume
493
issue
3
pages
448 - 459
publisher
John Wiley & Sons
external identifiers
  • pmid:16261535
  • wos:000233390400009
  • scopus:27944436547
ISSN
1096-9861
DOI
10.1002/cne.20766
language
English
LU publication?
yes
id
8abd20dd-9ed0-4062-8f8d-f17531af566c (old id 212142)
date added to LUP
2007-08-08 11:31:39
date last changed
2017-01-01 05:12:31
@article{8abd20dd-9ed0-4062-8f8d-f17531af566c,
  abstract     = {Although many effects of gamma-aminobutyric acid (GABA) on retinal function have been attributed to GABA(A) and GABA(C) receptors, specific retinal functions have also been shown to be mediated by GABA(B) receptors, including facilitation of light-evoked acetylcholine release from the rabbit retina (Neal and Cunningham [1995] J. Physiol. 482:363-372). To explain the results of a rich set of experiments, Neal and Cunningham proposed a model for this facilitation. In this model, GABA(B) receptor-mediated inhibition of glycinergic cells would reduce their own inhibition of cholinergic cells. In turn, muscarinic input from the latter to the glycinergic cells would complete a negative-feedback circuitry. In this study, we have used immunohistochemical techniques to test elements of this model. We report that glycinergic amacrine cells are GABA(B) receptor negative. In contrast, our data reveal the localization of GABA(B) receptors on cholinergic/GABAergic starburst amacrine cells. High-resolution localization of GABA(B) receptors on starburst amacrine cells shows that they are discretely localized to a limited population of its varicosities, the majority of likely synaptic-release sites being devoid of detectable levels of GABA(B) receptors. Finally, we identify a glycinergic cell that is a potential muscarinic receptor-bearing target of GABA(B)-modulated acetylcholine release. This target is the DAPI-3 cell. We propose, based on these data, a modification of the Neal and Cunningham model in which GABA(B) receptors are on starburst, not glycinergic amacrine cells.},
  author       = {Zucker, C L and Nilson, J E and Ehinger, Berndt and Grzywacz, N M},
  issn         = {1096-9861},
  keyword      = {directional selectivity,receptor,muscarinic,ganglion cell,amacrine cell,acetylcholine,glycine,receptor targeting},
  language     = {eng},
  number       = {3},
  pages        = {448--459},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Comparative Neurology},
  title        = {Compartmental localization of gamma-aminobutyric acid type B receptors in the cholinergic circuitry of the rabbit retina},
  url          = {http://dx.doi.org/10.1002/cne.20766},
  volume       = {493},
  year         = {2005},
}