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Non-specific effects of leukotriene synthesis inhibitors on HeLa cell physiology

Sabirsh, A; Bristulf, Jesper LU ; Karlsson, Ulf LU ; Owman, Christer LU and Haeggstrom, JZ (2005) In Prostaglandins, Leukotrienes and Essential Fatty Acids 73(6). p.431-440
Abstract
We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT1. All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT1, but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT1 expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes... (More)
We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT1. All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT1, but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT1 expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes have been reported to be important for aspects of the carcinogenic phenotype. We find that leukotriene synthesis inhibitors have non-specific effects, so careful controls are necessary to avoid interpreting non-specific effects as evidence for leukotriene involvement. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Prostaglandins, Leukotrienes and Essential Fatty Acids
volume
73
issue
6
pages
431 - 440
publisher
Elsevier
external identifiers
  • wos:000233369300004
  • pmid:16171988
  • scopus:27644483584
ISSN
0952-3278
DOI
10.1016/j.plefa.2005.08.004
language
English
LU publication?
yes
id
204b9040-5dd5-4700-9e7a-aeabe0b86674 (old id 212546)
date added to LUP
2007-08-20 13:59:00
date last changed
2017-01-01 04:30:41
@article{204b9040-5dd5-4700-9e7a-aeabe0b86674,
  abstract     = {We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT1. All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT1, but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT1 expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes have been reported to be important for aspects of the carcinogenic phenotype. We find that leukotriene synthesis inhibitors have non-specific effects, so careful controls are necessary to avoid interpreting non-specific effects as evidence for leukotriene involvement.},
  author       = {Sabirsh, A and Bristulf, Jesper and Karlsson, Ulf and Owman, Christer and Haeggstrom, JZ},
  issn         = {0952-3278},
  language     = {eng},
  number       = {6},
  pages        = {431--440},
  publisher    = {Elsevier},
  series       = {Prostaglandins, Leukotrienes and Essential Fatty Acids},
  title        = {Non-specific effects of leukotriene synthesis inhibitors on HeLa cell physiology},
  url          = {http://dx.doi.org/10.1016/j.plefa.2005.08.004},
  volume       = {73},
  year         = {2005},
}