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Structural conservation of the salivary gland-specific slalom gene in the blowfly Lucilia sericata

Ali, Reda LU ; Mellenthin, Katja LU ; Fahmy, Khalid LU ; Da Rocha Baez, Sol LU and Baumgartner, Stefan LU (2005) In Development Genes and Evolution 215(10). p.537-543
Abstract
Glycosylation and sulfation are two of the essential post-translational modifications of proteins. The slalom gene encodes a 3'-phosphoadenosine 5'-phosphosulfate transporter, a conserved protein found in organisms as diverse as plants and humans and required for sulfation of proteins. In Drosophila, slalom is exclusively expressed in salivary glands, which is unexpected, taken into account the general function for sulfation of proteins. In this paper, we present a detailed description of the slalom gene in a large insect, the blowfly Lucilia sericata. Our data demonstrate that the slalom gene structure, the protein and the expression pattern are highly conserved between Lucilia and Drosophila. Lucilia slalom promoter analysis, using... (More)
Glycosylation and sulfation are two of the essential post-translational modifications of proteins. The slalom gene encodes a 3'-phosphoadenosine 5'-phosphosulfate transporter, a conserved protein found in organisms as diverse as plants and humans and required for sulfation of proteins. In Drosophila, slalom is exclusively expressed in salivary glands, which is unexpected, taken into account the general function for sulfation of proteins. In this paper, we present a detailed description of the slalom gene in a large insect, the blowfly Lucilia sericata. Our data demonstrate that the slalom gene structure, the protein and the expression pattern are highly conserved between Lucilia and Drosophila. Lucilia slalom promoter analysis, using transgenic Drosophila, demonstrates that the Lucilia slalom promoter can faithfully mimic the expression pattern of both Lucilia and Drosophila slalom in salivary glands. Taken together, these data show the structure and the transcriptional cis-regulatory elements of the slalom gene to be unchanged during evolution, despite the 100 million years of divergence between the two insects. Moreover, it suggests that the salivary gland-specific expression of slalom bears an important and conserved function for sulfation of specific macromolecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
conservation, PAPS transporter, salivary gland, Lucilia sericata, Slalom
in
Development Genes and Evolution
volume
215
issue
10
pages
537 - 543
publisher
Springer
external identifiers
  • pmid:16003524
  • wos:000232990000006
  • scopus:27944478237
ISSN
0949-944X
DOI
10.1007/s00427-005-0010-2
language
English
LU publication?
yes
id
39e37efa-da03-40fe-9f69-4a3caab08114 (old id 214294)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16003524&dopt=Abstract
date added to LUP
2007-08-24 11:08:56
date last changed
2017-01-15 04:19:32
@article{39e37efa-da03-40fe-9f69-4a3caab08114,
  abstract     = {Glycosylation and sulfation are two of the essential post-translational modifications of proteins. The slalom gene encodes a 3'-phosphoadenosine 5'-phosphosulfate transporter, a conserved protein found in organisms as diverse as plants and humans and required for sulfation of proteins. In Drosophila, slalom is exclusively expressed in salivary glands, which is unexpected, taken into account the general function for sulfation of proteins. In this paper, we present a detailed description of the slalom gene in a large insect, the blowfly Lucilia sericata. Our data demonstrate that the slalom gene structure, the protein and the expression pattern are highly conserved between Lucilia and Drosophila. Lucilia slalom promoter analysis, using transgenic Drosophila, demonstrates that the Lucilia slalom promoter can faithfully mimic the expression pattern of both Lucilia and Drosophila slalom in salivary glands. Taken together, these data show the structure and the transcriptional cis-regulatory elements of the slalom gene to be unchanged during evolution, despite the 100 million years of divergence between the two insects. Moreover, it suggests that the salivary gland-specific expression of slalom bears an important and conserved function for sulfation of specific macromolecules.},
  author       = {Ali, Reda and Mellenthin, Katja and Fahmy, Khalid and Da Rocha Baez, Sol and Baumgartner, Stefan},
  issn         = {0949-944X},
  keyword      = {conservation,PAPS transporter,salivary gland,Lucilia sericata,Slalom},
  language     = {eng},
  number       = {10},
  pages        = {537--543},
  publisher    = {Springer},
  series       = {Development Genes and Evolution},
  title        = {Structural conservation of the salivary gland-specific slalom gene in the blowfly Lucilia sericata},
  url          = {http://dx.doi.org/10.1007/s00427-005-0010-2},
  volume       = {215},
  year         = {2005},
}