Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
(2021) In STAR Protocols 2(2). p.1-20- Abstract
Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/21507388-25cd-4592-b211-2831de88946e
- author
- Calvo-Garrido, Javier ; Winn, Dania ; Maffezzini, Camilla ; Wedell, Anna ; Freyer, Christoph ; Falk, Anna LU and Wredenberg, Anna
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- STAR Protocols
- volume
- 2
- issue
- 2
- article number
- 100528
- pages
- 1 - 20
- publisher
- Cell Press
- external identifiers
-
- pmid:34027486
- scopus:85105270013
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2021.100528
- language
- English
- LU publication?
- no
- id
- 21507388-25cd-4592-b211-2831de88946e
- date added to LUP
- 2021-08-09 12:59:49
- date last changed
- 2024-06-16 16:31:38
@article{21507388-25cd-4592-b211-2831de88946e,
abstract = {{<p>Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).</p>}},
author = {{Calvo-Garrido, Javier and Winn, Dania and Maffezzini, Camilla and Wedell, Anna and Freyer, Christoph and Falk, Anna and Wredenberg, Anna}},
issn = {{2666-1667}},
language = {{eng}},
number = {{2}},
pages = {{1--20}},
publisher = {{Cell Press}},
series = {{STAR Protocols}},
title = {{Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro}},
url = {{https://lup.lub.lu.se/search/files/101029371/Protocol_for_the_derivation.pdf}},
doi = {{10.1016/j.xpro.2021.100528}},
volume = {{2}},
year = {{2021}},
}