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Rho Kinase-related Proteins in Human Vaginal Arteries: An Immunohistochemical and Functional Study

Uckert, Stefan; Waldkirch, Eginhard S; Kauffels, Wolfgang; Kuczyk, Markus A and Hedlund, Petter LU (2011) In Journal of Sexual Medicine 8(10). p.2739-2745
Abstract
The calcium-sensitizing Rho A/Rho kinase pathway has been suggested to play a role in the control of nongenital vascular smooth muscle. Rho-associated kinases (ROKs) cause calcium-independent modulation of smooth muscle contraction, and have been demonstrated in the bladder, prostate, and corpus cavernosum. Until now, it is not known whether ROKs and related proteins play a role in the control of vaginal blood flow. Aim.  To investigate by means of functional studies and immunohistochemistry the significance of the Rho pathway in human vaginal arteries. Methods.  Vaginal tissue was obtained from five postmenopausal women. Specimens were processed for immunohistochemistry for ROK1, ROK2, RhoA, and RhoGDI. Segments of sub-epithelial vaginal... (More)
The calcium-sensitizing Rho A/Rho kinase pathway has been suggested to play a role in the control of nongenital vascular smooth muscle. Rho-associated kinases (ROKs) cause calcium-independent modulation of smooth muscle contraction, and have been demonstrated in the bladder, prostate, and corpus cavernosum. Until now, it is not known whether ROKs and related proteins play a role in the control of vaginal blood flow. Aim.  To investigate by means of functional studies and immunohistochemistry the significance of the Rho pathway in human vaginal arteries. Methods.  Vaginal tissue was obtained from five postmenopausal women. Specimens were processed for immunohistochemistry for ROK1, ROK2, RhoA, and RhoGDI. Segments of sub-epithelial vaginal arteries were mounted in a tissue bath. Effects of Y27632 on the concentration-response curves to phenylephrine (Phe) or Phe-precontracted preparations were investigated. Main Outcome Measure.  The expression of Rho kinases ROK1, ROK2, and the Rho-associated protein RhoGDI in human vaginal arteries was investigated by means of immunohistochemistry. Tissue bath studies were conducted in order to characterize the effects of the ROK inhibitor Y27632 on isolated vaginal arteries. Results.  A meshwork of α-actin immunoreactive arterioles was located in the sub-epithelium of human vaginal specimens. Immunoreactivities for ROK1, ROK2, RhoA, and RhoGDI were expressed in the smooth musculature of these arteries. At 0.1 and 1 µM Y27632, the contraction to Phe (10 µM) was 99 ± 17% and 28 ± 12% that of 124 mM K(+) . In Phe-contracted preparations, Y27632 produced relaxant responses. Conclusions.  The activation of alpha(1) -adrenoceptors contracts sub-epithelial human vaginal arteries via ROK-sensitive mechanisms. A role for these signals in the regulation of vaginal blood flow might be considered (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Rho-related Proteins, Human Vaginal Arteries, Rho Kinases, Immunohistochemistry, Tissue Bath Studies, Vaginal Blood Flow
in
Journal of Sexual Medicine
volume
8
issue
10
pages
2739 - 2745
publisher
Wiley-Blackwell
external identifiers
  • wos:000295874900008
  • pmid:21797982
  • scopus:80053564380
ISSN
1743-6109
DOI
10.1111/j.1743-6109.2011.02390.x
language
English
LU publication?
yes
id
5899a70b-9999-4f92-b924-d6a38d2da800 (old id 2151736)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21797982?dopt=Abstract
date added to LUP
2011-09-04 20:59:43
date last changed
2017-01-01 03:49:22
@article{5899a70b-9999-4f92-b924-d6a38d2da800,
  abstract     = {The calcium-sensitizing Rho A/Rho kinase pathway has been suggested to play a role in the control of nongenital vascular smooth muscle. Rho-associated kinases (ROKs) cause calcium-independent modulation of smooth muscle contraction, and have been demonstrated in the bladder, prostate, and corpus cavernosum. Until now, it is not known whether ROKs and related proteins play a role in the control of vaginal blood flow. Aim.  To investigate by means of functional studies and immunohistochemistry the significance of the Rho pathway in human vaginal arteries. Methods.  Vaginal tissue was obtained from five postmenopausal women. Specimens were processed for immunohistochemistry for ROK1, ROK2, RhoA, and RhoGDI. Segments of sub-epithelial vaginal arteries were mounted in a tissue bath. Effects of Y27632 on the concentration-response curves to phenylephrine (Phe) or Phe-precontracted preparations were investigated. Main Outcome Measure.  The expression of Rho kinases ROK1, ROK2, and the Rho-associated protein RhoGDI in human vaginal arteries was investigated by means of immunohistochemistry. Tissue bath studies were conducted in order to characterize the effects of the ROK inhibitor Y27632 on isolated vaginal arteries. Results.  A meshwork of α-actin immunoreactive arterioles was located in the sub-epithelium of human vaginal specimens. Immunoreactivities for ROK1, ROK2, RhoA, and RhoGDI were expressed in the smooth musculature of these arteries. At 0.1 and 1 µM Y27632, the contraction to Phe (10 µM) was 99 ± 17% and 28 ± 12% that of 124 mM K(+) . In Phe-contracted preparations, Y27632 produced relaxant responses. Conclusions.  The activation of alpha(1) -adrenoceptors contracts sub-epithelial human vaginal arteries via ROK-sensitive mechanisms. A role for these signals in the regulation of vaginal blood flow might be considered},
  author       = {Uckert, Stefan and Waldkirch, Eginhard S and Kauffels, Wolfgang and Kuczyk, Markus A and Hedlund, Petter},
  issn         = {1743-6109},
  keyword      = {Rho-related Proteins,Human Vaginal Arteries,Rho Kinases,Immunohistochemistry,Tissue Bath Studies,Vaginal Blood Flow},
  language     = {eng},
  number       = {10},
  pages        = {2739--2745},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Sexual Medicine},
  title        = {Rho Kinase-related Proteins in Human Vaginal Arteries: An Immunohistochemical and Functional Study},
  url          = {http://dx.doi.org/10.1111/j.1743-6109.2011.02390.x},
  volume       = {8},
  year         = {2011},
}