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Immunophenotypical characterization of human neutrophil differentiation

Mora-Jensen, Helena; Jendholm, Johan; Fossum, Anna; Porse, Bo; Borregaad, Niels and Theilgaard-Moench, Kim LU (2011) In Journal of Leukocyte Biology 90(3). p.629-634
Abstract
The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid... (More)
The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs. J. Leukoc. Biol. 90: 629-634; 2011. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
granulocytes, myeloid differentiation, differentiation pathway, flow, cytometry, human bone marrow
in
Journal of Leukocyte Biology
volume
90
issue
3
pages
629 - 634
publisher
Society for Leukocyte Biology
external identifiers
  • wos:000294441700023
  • scopus:80052364253
ISSN
1938-3673
DOI
10.1189/jlb.0311123
language
English
LU publication?
yes
id
4fa517d2-e12d-43b0-9f57-a4a0b05ab6a6 (old id 2159335)
date added to LUP
2011-10-03 08:29:38
date last changed
2017-10-01 03:26:25
@article{4fa517d2-e12d-43b0-9f57-a4a0b05ab6a6,
  abstract     = {The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs. J. Leukoc. Biol. 90: 629-634; 2011.},
  author       = {Mora-Jensen, Helena and Jendholm, Johan and Fossum, Anna and Porse, Bo and Borregaad, Niels and Theilgaard-Moench, Kim},
  issn         = {1938-3673},
  keyword      = {granulocytes,myeloid differentiation,differentiation pathway,flow,cytometry,human bone marrow},
  language     = {eng},
  number       = {3},
  pages        = {629--634},
  publisher    = {Society for Leukocyte Biology},
  series       = {Journal of Leukocyte Biology},
  title        = {Immunophenotypical characterization of human neutrophil differentiation},
  url          = {http://dx.doi.org/10.1189/jlb.0311123},
  volume       = {90},
  year         = {2011},
}