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Determinants of stimulated peripheral blood cytokine production among farming women

Lampi, Jussi; Roponen, Marjut; Hyvarinen, Anne; Hirvonen, Maija-Riitta; Larsson, Lennart LU ; Nevalainen, Aino and Pekkanen, Juha (2011) In International Journal of Hygiene and Environmental Health 214(3). p.205-209
Abstract
Farming environment and environmental exposure to microbial agents have been suggested to promote favorable development of immune system in children and protect against allergic diseases. However, effects of farm exposure on adult immune responses are less clear. Aim of the present study was to examine associations of farm related factors and measured microbial exposure with stimulated production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in peripheral blood samples among farming women. Whole peripheral blood samples were obtained from 112 women living on farms and stimulated with phorbol myristate acetate/ionomycin, lipopolysaccharide and staphylococcal enterotoxin B. Following 24 h stimulation, protein levels of IFN-gamma... (More)
Farming environment and environmental exposure to microbial agents have been suggested to promote favorable development of immune system in children and protect against allergic diseases. However, effects of farm exposure on adult immune responses are less clear. Aim of the present study was to examine associations of farm related factors and measured microbial exposure with stimulated production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in peripheral blood samples among farming women. Whole peripheral blood samples were obtained from 112 women living on farms and stimulated with phorbol myristate acetate/ionomycin, lipopolysaccharide and staphylococcal enterotoxin B. Following 24 h stimulation, protein levels of IFN-gamma and IL-4 in the supernatants were measured by ELISA. From house dust, concentrations of 3-hydroxy fatty acids (C10:0-C14:0, marker for Gram-negative bacteria), muramic acid (Gram-positive bacteria) and ergosterol (fungal biomass) were analyzed with GC-MS/MS and viable microbes by culturing. Information on farm related factors and allergic diseases were collected from self-administered questionnaires. We found that household pets or other current or childhood farm-related factors had only few associations with stimulated cytokine production among studied farming women. Similarly, no strong associations were observed between markers of microbial exposure measured in house dust and cytokine levels. Atopic sensitization, allergic rhinitis and recent respiratory infections were, however, associated with reduced IFN-gamma production. Our results suggest that the capacity of the studied environmental factors to modulate immune system is relatively weak in adulthood. (C) 2011 Elsevier GmbH. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Farming, House dust, Interferon-gamma, Interleukin-4, Microbes
in
International Journal of Hygiene and Environmental Health
volume
214
issue
3
pages
205 - 209
publisher
Elsevier
external identifiers
  • wos:000293814600004
  • scopus:79958056644
ISSN
1618-131X
DOI
10.1016/j.ijheh.2011.01.007
language
English
LU publication?
yes
id
b2ee23f3-fe08-4d9c-8e8f-07e6a6e0ce08 (old id 2162003)
date added to LUP
2011-10-03 08:37:02
date last changed
2017-01-01 06:00:17
@article{b2ee23f3-fe08-4d9c-8e8f-07e6a6e0ce08,
  abstract     = {Farming environment and environmental exposure to microbial agents have been suggested to promote favorable development of immune system in children and protect against allergic diseases. However, effects of farm exposure on adult immune responses are less clear. Aim of the present study was to examine associations of farm related factors and measured microbial exposure with stimulated production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in peripheral blood samples among farming women. Whole peripheral blood samples were obtained from 112 women living on farms and stimulated with phorbol myristate acetate/ionomycin, lipopolysaccharide and staphylococcal enterotoxin B. Following 24 h stimulation, protein levels of IFN-gamma and IL-4 in the supernatants were measured by ELISA. From house dust, concentrations of 3-hydroxy fatty acids (C10:0-C14:0, marker for Gram-negative bacteria), muramic acid (Gram-positive bacteria) and ergosterol (fungal biomass) were analyzed with GC-MS/MS and viable microbes by culturing. Information on farm related factors and allergic diseases were collected from self-administered questionnaires. We found that household pets or other current or childhood farm-related factors had only few associations with stimulated cytokine production among studied farming women. Similarly, no strong associations were observed between markers of microbial exposure measured in house dust and cytokine levels. Atopic sensitization, allergic rhinitis and recent respiratory infections were, however, associated with reduced IFN-gamma production. Our results suggest that the capacity of the studied environmental factors to modulate immune system is relatively weak in adulthood. (C) 2011 Elsevier GmbH. All rights reserved.},
  author       = {Lampi, Jussi and Roponen, Marjut and Hyvarinen, Anne and Hirvonen, Maija-Riitta and Larsson, Lennart and Nevalainen, Aino and Pekkanen, Juha},
  issn         = {1618-131X},
  keyword      = {Farming,House dust,Interferon-gamma,Interleukin-4,Microbes},
  language     = {eng},
  number       = {3},
  pages        = {205--209},
  publisher    = {Elsevier},
  series       = {International Journal of Hygiene and Environmental Health},
  title        = {Determinants of stimulated peripheral blood cytokine production among farming women},
  url          = {http://dx.doi.org/10.1016/j.ijheh.2011.01.007},
  volume       = {214},
  year         = {2011},
}