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FLT3 expression initiates in fully multipotent mouse hematopoietic progenitor cells

Buza-Vidas, Natalija; Woll, Petter; Hultquist, Anne LU ; Duarte, Sara; Lutteropp, Michael; Bouriez-Jones, Tiphaine; Ferry, Helen; Luc, Sidinh LU and Jacobsen, Sten Eirik W LU (2011) In Blood 118(6). p.1544-1548
Abstract
Lymphoid-primed multipotent progenitors with down-regulated megakaryocyte-erythroid (MkE) potential are restricted to cells with high levels of cell-surface FLT3 expression, whereas HSCs and MkE progenitors lack detectable cell-surface FLT3. These findings are compatible with FLT3 cell-surface expression not being detectable in the fully multipotent stem/progenitor cell compartment in mice. If so, this process could be distinct from human hematopoiesis, in which FLT3 already is expressed in multipotent stem/progenitor cells. The expression pattern of Flt3 (mRNA) and FLT3 (protein) in multipotent progenitors is of considerable relevance for mouse models in which prognostically important Flt3 mutations are expressed under control of the... (More)
Lymphoid-primed multipotent progenitors with down-regulated megakaryocyte-erythroid (MkE) potential are restricted to cells with high levels of cell-surface FLT3 expression, whereas HSCs and MkE progenitors lack detectable cell-surface FLT3. These findings are compatible with FLT3 cell-surface expression not being detectable in the fully multipotent stem/progenitor cell compartment in mice. If so, this process could be distinct from human hematopoiesis, in which FLT3 already is expressed in multipotent stem/progenitor cells. The expression pattern of Flt3 (mRNA) and FLT3 (protein) in multipotent progenitors is of considerable relevance for mouse models in which prognostically important Flt3 mutations are expressed under control of the endogenous mouse Flt3 promoter. Herein, we demonstrate that mouse Flt3 expression initiates in fully multipotent progenitors because in addition to lymphoid and granulocyte-monocyte progenitors, FLT3(-) Mk- and E-restricted downstream progenitors are also highly labeled when Flt3-Cre fate mapping is applied. (Blood. 2011;118(6):1544-1548) (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
118
issue
6
pages
1544 - 1548
publisher
American Society of Hematology
external identifiers
  • wos:000293787300021
  • scopus:80051606868
ISSN
1528-0020
DOI
10.1182/blood-2010-10-316232
language
English
LU publication?
yes
id
545cb561-63f1-44f3-9aee-3bae1de410f9 (old id 2162143)
date added to LUP
2011-10-03 08:38:14
date last changed
2017-07-23 03:27:18
@article{545cb561-63f1-44f3-9aee-3bae1de410f9,
  abstract     = {Lymphoid-primed multipotent progenitors with down-regulated megakaryocyte-erythroid (MkE) potential are restricted to cells with high levels of cell-surface FLT3 expression, whereas HSCs and MkE progenitors lack detectable cell-surface FLT3. These findings are compatible with FLT3 cell-surface expression not being detectable in the fully multipotent stem/progenitor cell compartment in mice. If so, this process could be distinct from human hematopoiesis, in which FLT3 already is expressed in multipotent stem/progenitor cells. The expression pattern of Flt3 (mRNA) and FLT3 (protein) in multipotent progenitors is of considerable relevance for mouse models in which prognostically important Flt3 mutations are expressed under control of the endogenous mouse Flt3 promoter. Herein, we demonstrate that mouse Flt3 expression initiates in fully multipotent progenitors because in addition to lymphoid and granulocyte-monocyte progenitors, FLT3(-) Mk- and E-restricted downstream progenitors are also highly labeled when Flt3-Cre fate mapping is applied. (Blood. 2011;118(6):1544-1548)},
  author       = {Buza-Vidas, Natalija and Woll, Petter and Hultquist, Anne and Duarte, Sara and Lutteropp, Michael and Bouriez-Jones, Tiphaine and Ferry, Helen and Luc, Sidinh and Jacobsen, Sten Eirik W},
  issn         = {1528-0020},
  language     = {eng},
  number       = {6},
  pages        = {1544--1548},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {FLT3 expression initiates in fully multipotent mouse hematopoietic progenitor cells},
  url          = {http://dx.doi.org/10.1182/blood-2010-10-316232},
  volume       = {118},
  year         = {2011},
}