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Experimental vitreous tamponade using polyalkylimide hydrogel

Crafoord, Sven; Andréasson, Sten LU and Ghosh, Fredrik LU (2011) In Graefe's Archive for Clinical and Experimental Ophthalmology 249(8). p.1167-1174
Abstract
To evaluate polyalkylimide as a possible vitreous tamponading agent. A 20-gauge pars plana vitrectomy and posterior vitreous detachment were performed in the right eye of six pigmented rabbits. Approximately 1 ml of viscoelastic gel, polyalkylimide (Bio-AlcamidA (R)) was thereafter injected into the vitreous space. Full-field ERG and intraocular pressure (IOP, Tonopen) was measured pre-and postoperatively at regular intervals up to 28 days. At day 6 or 28, the rabbits were sacrificed and the eyes were examined macroscopically, photographed, and prepared for histological examination with routine microscopy. The viscoelastic hydrogel was successfully injected, and remained translucent with preserved gel properties throughout the... (More)
To evaluate polyalkylimide as a possible vitreous tamponading agent. A 20-gauge pars plana vitrectomy and posterior vitreous detachment were performed in the right eye of six pigmented rabbits. Approximately 1 ml of viscoelastic gel, polyalkylimide (Bio-AlcamidA (R)) was thereafter injected into the vitreous space. Full-field ERG and intraocular pressure (IOP, Tonopen) was measured pre-and postoperatively at regular intervals up to 28 days. At day 6 or 28, the rabbits were sacrificed and the eyes were examined macroscopically, photographed, and prepared for histological examination with routine microscopy. The viscoelastic hydrogel was successfully injected, and remained translucent with preserved gel properties throughout the postoperative period. The postoperative IOP was unchanged compared to preoperative values. Five of six eyes displayed retinal edema or pigmentary changes centrally while the periphery appeared intact. ERG recordings showed a radical decrease in rod- and cone-derived B-wave amplitudes. Histological examination confirmed varying degrees of edema combined with neuronal cell death within the retinal layers in the central part of the fundus, while the peripheral part appeared intact. Polyalkylimide displays favourable physical properties when used as a vitreous tamponade. However, the hydrogel causes functional and morphological retinal damage when in direct contact with the inner retina. Possible pathological mechanisms include osmotic imbalance and direct toxic effects, and modification of biochemical properties is warranted before clinical use will be possible. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Vitreous, Vitrectomy, Photoreceptor death
in
Graefe's Archive for Clinical and Experimental Ophthalmology
volume
249
issue
8
pages
1167 - 1174
publisher
Springer
external identifiers
  • wos:000293642300008
  • scopus:80052713545
ISSN
1435-702X
DOI
10.1007/s00417-011-1652-6
language
English
LU publication?
yes
id
58acc7e3-57b9-4465-93cc-a3838baf0729 (old id 2162979)
date added to LUP
2011-10-03 08:39:05
date last changed
2017-10-22 04:19:41
@article{58acc7e3-57b9-4465-93cc-a3838baf0729,
  abstract     = {To evaluate polyalkylimide as a possible vitreous tamponading agent. A 20-gauge pars plana vitrectomy and posterior vitreous detachment were performed in the right eye of six pigmented rabbits. Approximately 1 ml of viscoelastic gel, polyalkylimide (Bio-AlcamidA (R)) was thereafter injected into the vitreous space. Full-field ERG and intraocular pressure (IOP, Tonopen) was measured pre-and postoperatively at regular intervals up to 28 days. At day 6 or 28, the rabbits were sacrificed and the eyes were examined macroscopically, photographed, and prepared for histological examination with routine microscopy. The viscoelastic hydrogel was successfully injected, and remained translucent with preserved gel properties throughout the postoperative period. The postoperative IOP was unchanged compared to preoperative values. Five of six eyes displayed retinal edema or pigmentary changes centrally while the periphery appeared intact. ERG recordings showed a radical decrease in rod- and cone-derived B-wave amplitudes. Histological examination confirmed varying degrees of edema combined with neuronal cell death within the retinal layers in the central part of the fundus, while the peripheral part appeared intact. Polyalkylimide displays favourable physical properties when used as a vitreous tamponade. However, the hydrogel causes functional and morphological retinal damage when in direct contact with the inner retina. Possible pathological mechanisms include osmotic imbalance and direct toxic effects, and modification of biochemical properties is warranted before clinical use will be possible.},
  author       = {Crafoord, Sven and Andréasson, Sten and Ghosh, Fredrik},
  issn         = {1435-702X},
  keyword      = {Vitreous,Vitrectomy,Photoreceptor death},
  language     = {eng},
  number       = {8},
  pages        = {1167--1174},
  publisher    = {Springer},
  series       = {Graefe's Archive for Clinical and Experimental Ophthalmology},
  title        = {Experimental vitreous tamponade using polyalkylimide hydrogel},
  url          = {http://dx.doi.org/10.1007/s00417-011-1652-6},
  volume       = {249},
  year         = {2011},
}