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B-lymphocyte commitment: Identifying the point of no return.

Welinder, Eva LU ; Ahsberg, Josefine and Sigvardsson, Mikael LU (2011) In Seminars in Immunology 23. p.335-340
Abstract
Even though B-lymphocyte development is one of the best understood models for cell differentiation in the hematopoetic system, recent advances in cell sorting and functional genomics has increased this understanding further. This has suggested that already early lymphoid primed multipotent progenitor cells (LMPPs) express low levels of lymphoid restricted transcripts. The expression of these genes becomes more pronounced when cells enter the FLT-3/IL-7 receptor positive common lymphoid progenitor (CLP) stage. However, the expression of B-lineage specific genes is limited to a B-cell restricted Ly6D surface positive subpopulation of the CLP compartment. The gene expression patterns also reflect differences in lineage potential and while... (More)
Even though B-lymphocyte development is one of the best understood models for cell differentiation in the hematopoetic system, recent advances in cell sorting and functional genomics has increased this understanding further. This has suggested that already early lymphoid primed multipotent progenitor cells (LMPPs) express low levels of lymphoid restricted transcripts. The expression of these genes becomes more pronounced when cells enter the FLT-3/IL-7 receptor positive common lymphoid progenitor (CLP) stage. However, the expression of B-lineage specific genes is limited to a B-cell restricted Ly6D surface positive subpopulation of the CLP compartment. The gene expression patterns also reflect differences in lineage potential and while Ly6D negative FLT-3/IL-7 receptor positive cells represents true CLPs with an ability to generate B/T and NK cells, the Ly6D positive cells lack NK cell potential and display a reduced T-cell potential in vivo. These recent findings suggest that the CLP compartment is highly heterogenous and that the point of no return in B-cell development may occur already in B220(-)CD19(-) cells. These findings have allowed for a better understanding of the interplay between transcription factors like EBF-1, PAX-5 and E47, all known as crucial for normal B-cell development. In this review, we aim to provide a comprehensive overview of B-cell fate specification and commitment based on the recent advances in the understanding of molecular networks as well as functional properties of early progenitor populations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Seminars in Immunology
volume
23
pages
335 - 340
publisher
Elsevier
external identifiers
  • wos:000297447300004
  • pmid:21944938
  • scopus:82555179207
ISSN
1096-3618
DOI
10.1016/j.smim.2011.08.005
language
English
LU publication?
yes
id
5c9c3efb-47ab-49a1-bb05-82fe36345ff4 (old id 2168431)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21944938?dopt=Abstract
date added to LUP
2011-10-03 14:14:43
date last changed
2017-03-26 04:31:50
@article{5c9c3efb-47ab-49a1-bb05-82fe36345ff4,
  abstract     = {Even though B-lymphocyte development is one of the best understood models for cell differentiation in the hematopoetic system, recent advances in cell sorting and functional genomics has increased this understanding further. This has suggested that already early lymphoid primed multipotent progenitor cells (LMPPs) express low levels of lymphoid restricted transcripts. The expression of these genes becomes more pronounced when cells enter the FLT-3/IL-7 receptor positive common lymphoid progenitor (CLP) stage. However, the expression of B-lineage specific genes is limited to a B-cell restricted Ly6D surface positive subpopulation of the CLP compartment. The gene expression patterns also reflect differences in lineage potential and while Ly6D negative FLT-3/IL-7 receptor positive cells represents true CLPs with an ability to generate B/T and NK cells, the Ly6D positive cells lack NK cell potential and display a reduced T-cell potential in vivo. These recent findings suggest that the CLP compartment is highly heterogenous and that the point of no return in B-cell development may occur already in B220(-)CD19(-) cells. These findings have allowed for a better understanding of the interplay between transcription factors like EBF-1, PAX-5 and E47, all known as crucial for normal B-cell development. In this review, we aim to provide a comprehensive overview of B-cell fate specification and commitment based on the recent advances in the understanding of molecular networks as well as functional properties of early progenitor populations.},
  author       = {Welinder, Eva and Ahsberg, Josefine and Sigvardsson, Mikael},
  issn         = {1096-3618},
  language     = {eng},
  pages        = {335--340},
  publisher    = {Elsevier},
  series       = {Seminars in Immunology},
  title        = {B-lymphocyte commitment: Identifying the point of no return.},
  url          = {http://dx.doi.org/10.1016/j.smim.2011.08.005},
  volume       = {23},
  year         = {2011},
}