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Analysis of vesicular trafficking in primary neurons by live imaging.

Tampellini, Davide LU and Gouras, Gunnar LU (2011) In Methods in Molecular Biology 793. p.343-350
Abstract
Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
793
pages
343 - 350
publisher
Springer
external identifiers
  • pmid:21913111
  • scopus:80054751590
ISSN
1940-6029
DOI
10.1007/978-1-61779-328-8_22
language
English
LU publication?
yes
id
641b8a78-22c6-4150-866c-4bf065961436 (old id 2168939)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21913111?dopt=Abstract
date added to LUP
2011-10-03 10:48:51
date last changed
2017-01-01 07:41:00
@article{641b8a78-22c6-4150-866c-4bf065961436,
  abstract     = {Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.},
  author       = {Tampellini, Davide and Gouras, Gunnar},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {343--350},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Analysis of vesicular trafficking in primary neurons by live imaging.},
  url          = {http://dx.doi.org/10.1007/978-1-61779-328-8_22},
  volume       = {793},
  year         = {2011},
}