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Recombinant antibodies for the generation of antibody arrays.

Borrebaeck, Carl LU and Wingren, Christer LU (2011) In Methods in Molecular Biology 785. p.247-262
Abstract
Affinity proteomics, mainly represented by antibody microarrays, has in recent years been established as a powerful tool for high-throughput (disease) proteomics. The technology can be used to generate detailed protein expression profiles, or protein maps, of focused set of proteins in crude proteomes and potentially even high-resolution portraits of entire proteomes. The technology provides unique opportunities, for example biomarker discovery, disease diagnostics, patient stratification and monitoring of disease, and taking the next steps toward personalized medicine. However, the process of designing high-performing, high-density antibody micro- and nanoarrays has proven to be challenging, requiring truly cross-disciplinary efforts to... (More)
Affinity proteomics, mainly represented by antibody microarrays, has in recent years been established as a powerful tool for high-throughput (disease) proteomics. The technology can be used to generate detailed protein expression profiles, or protein maps, of focused set of proteins in crude proteomes and potentially even high-resolution portraits of entire proteomes. The technology provides unique opportunities, for example biomarker discovery, disease diagnostics, patient stratification and monitoring of disease, and taking the next steps toward personalized medicine. However, the process of designing high-performing, high-density antibody micro- and nanoarrays has proven to be challenging, requiring truly cross-disciplinary efforts to be adopted. In this mini-review, we address one of these key technological issues, namely, the choice of probe format, and focus on the use of recombinant antibodies vs. polyclonal and monoclonal antibodies for the generation of antibody arrays. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
785
pages
247 - 262
publisher
Springer
external identifiers
  • pmid:21901605
  • scopus:80054735822
ISSN
1940-6029
DOI
10.1007/978-1-61779-286-1_17
language
English
LU publication?
yes
id
d74c24c8-d4cd-4287-bce2-a28e5539cb34 (old id 2169053)
date added to LUP
2011-09-30 09:32:05
date last changed
2017-08-06 03:06:06
@article{d74c24c8-d4cd-4287-bce2-a28e5539cb34,
  abstract     = {Affinity proteomics, mainly represented by antibody microarrays, has in recent years been established as a powerful tool for high-throughput (disease) proteomics. The technology can be used to generate detailed protein expression profiles, or protein maps, of focused set of proteins in crude proteomes and potentially even high-resolution portraits of entire proteomes. The technology provides unique opportunities, for example biomarker discovery, disease diagnostics, patient stratification and monitoring of disease, and taking the next steps toward personalized medicine. However, the process of designing high-performing, high-density antibody micro- and nanoarrays has proven to be challenging, requiring truly cross-disciplinary efforts to be adopted. In this mini-review, we address one of these key technological issues, namely, the choice of probe format, and focus on the use of recombinant antibodies vs. polyclonal and monoclonal antibodies for the generation of antibody arrays.},
  author       = {Borrebaeck, Carl and Wingren, Christer},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {247--262},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Recombinant antibodies for the generation of antibody arrays.},
  url          = {http://dx.doi.org/10.1007/978-1-61779-286-1_17},
  volume       = {785},
  year         = {2011},
}