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Systematic Screening of BK Virus by Real-Time PCR Prevents BK Virus Associated Nephropathy in Renal Transplant Recipients

Hammarin, Anna-Lena; Öqvist, Björn LU ; Wahlgren, John and Falk, Kerstin I. (2011) In Journal of Medical Virology 83(11). p.1959-1965
Abstract
BK virus associated nephropathy occurs in approximately 5% of renal transplant recipients. Quantitation of BKV DNA in serum/plasma early in the course of disease has been suggested to be an important diagnostic tool for polymavirus-associated nephropathy (PVAN). The aim of this study was to develop a BKV real-time PCR (qPCR), which could be included in a diagnostic qPCR platform. Additionally, the significance of the assay as a surrogate marker for PVAN was investigated. Quantitation of BKV DNA by qPCR was carried out on 234 serum samples from a retrospective study including 31 renal transplant recipients monitored for at least 6 months post-transplantation. BKV viremia was detected in 9 out of 31 patients. Four patients had a viral load... (More)
BK virus associated nephropathy occurs in approximately 5% of renal transplant recipients. Quantitation of BKV DNA in serum/plasma early in the course of disease has been suggested to be an important diagnostic tool for polymavirus-associated nephropathy (PVAN). The aim of this study was to develop a BKV real-time PCR (qPCR), which could be included in a diagnostic qPCR platform. Additionally, the significance of the assay as a surrogate marker for PVAN was investigated. Quantitation of BKV DNA by qPCR was carried out on 234 serum samples from a retrospective study including 31 renal transplant recipients monitored for at least 6 months post-transplantation. BKV viremia was detected in 9 out of 31 patients. Four patients had a viral load of >10,000 copies/ml at least on one occasion. In two of these patients, PVAN was diagnosed clinically during the study period. In retrospect, these patients were shown to be BKV positive before the clinical diagnosis of PVAN was made. Another two patients had a permanent graft dysfunction, but were never clinically diagnosed with PVAN. None of the remaining five patients with BKV DNA (<10,000 copies/ml) had renal impairment. Based on these results, an algorithm was introduced at the study center in 2006 and to date, August 2011, no cases of PVAN with loss of graft have been observed. The concept of including different PCR protocols in a common qPCR platform allows laboratories with small sample numbers to perform regularly a variety of assays at a reasonable cost. Med. Virol. 83:1959-1965, 2011. (C) 2011 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
viral load, polyoma virus, PVAN, qPCR platform
in
Journal of Medical Virology
volume
83
issue
11
pages
1959 - 1965
publisher
John Wiley & Sons
external identifiers
  • wos:000295196200013
  • scopus:80052711401
ISSN
1096-9071
DOI
10.1002/jmv.22196
language
English
LU publication?
yes
id
7c002a3e-3fea-4aad-ab98-9e27ba6b6de3 (old id 2179383)
date added to LUP
2011-11-01 07:54:12
date last changed
2017-01-01 03:15:49
@article{7c002a3e-3fea-4aad-ab98-9e27ba6b6de3,
  abstract     = {BK virus associated nephropathy occurs in approximately 5% of renal transplant recipients. Quantitation of BKV DNA in serum/plasma early in the course of disease has been suggested to be an important diagnostic tool for polymavirus-associated nephropathy (PVAN). The aim of this study was to develop a BKV real-time PCR (qPCR), which could be included in a diagnostic qPCR platform. Additionally, the significance of the assay as a surrogate marker for PVAN was investigated. Quantitation of BKV DNA by qPCR was carried out on 234 serum samples from a retrospective study including 31 renal transplant recipients monitored for at least 6 months post-transplantation. BKV viremia was detected in 9 out of 31 patients. Four patients had a viral load of &gt;10,000 copies/ml at least on one occasion. In two of these patients, PVAN was diagnosed clinically during the study period. In retrospect, these patients were shown to be BKV positive before the clinical diagnosis of PVAN was made. Another two patients had a permanent graft dysfunction, but were never clinically diagnosed with PVAN. None of the remaining five patients with BKV DNA (&lt;10,000 copies/ml) had renal impairment. Based on these results, an algorithm was introduced at the study center in 2006 and to date, August 2011, no cases of PVAN with loss of graft have been observed. The concept of including different PCR protocols in a common qPCR platform allows laboratories with small sample numbers to perform regularly a variety of assays at a reasonable cost. Med. Virol. 83:1959-1965, 2011. (C) 2011 Wiley-Liss, Inc.},
  author       = {Hammarin, Anna-Lena and Öqvist, Björn and Wahlgren, John and Falk, Kerstin I.},
  issn         = {1096-9071},
  keyword      = {viral load,polyoma virus,PVAN,qPCR platform},
  language     = {eng},
  number       = {11},
  pages        = {1959--1965},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Medical Virology},
  title        = {Systematic Screening of BK Virus by Real-Time PCR Prevents BK Virus Associated Nephropathy in Renal Transplant Recipients},
  url          = {http://dx.doi.org/10.1002/jmv.22196},
  volume       = {83},
  year         = {2011},
}