Advanced

The dual regulator Sufu integrates Hedgehog and Wnt signals in the early Xenopus embryo

Tan Grahn, Hooi Min LU ; Kriebel, Martin; Hou, Shirui and Pera, Edgar LU (2011) In Developmental Biology 358(1). p.262-276
Abstract
Hedgehog (Hh) and Wnt proteins are important signals implicated in several aspects of embryonic development, including the early development of the central nervous system. We found that Xenopus Suppressor-of-fused (XSufu) affects neural induction and patterning by regulating the Hh/Gli and Wnt/beta-catenin pathways. Microinjection of XSufu mRNA induced expansion of the epidermis at the expense of neural plate tissue and caused enlargement of the eyes. An antisense morpholino oligonucleotide against XSufu had the opposite effect. Interestingly, both gain- and loss-of-function experiments resulted in a posterior shift of brain markers, suggesting a biphasic effect of XSufu on anteroposterior patterning. XSufu blocked early Wnt/beta-catenin... (More)
Hedgehog (Hh) and Wnt proteins are important signals implicated in several aspects of embryonic development, including the early development of the central nervous system. We found that Xenopus Suppressor-of-fused (XSufu) affects neural induction and patterning by regulating the Hh/Gli and Wnt/beta-catenin pathways. Microinjection of XSufu mRNA induced expansion of the epidermis at the expense of neural plate tissue and caused enlargement of the eyes. An antisense morpholino oligonucleotide against XSufu had the opposite effect. Interestingly, both gain- and loss-of-function experiments resulted in a posterior shift of brain markers, suggesting a biphasic effect of XSufu on anteroposterior patterning. XSufu blocked early Wnt/beta-catenin signaling, as indicated by the suppression of XWnt8-induced secondary axis formation in mRNA-injected embryos, and activation of Wnt target genes in XSufu-MO-injected ectodermal explants. We show that XSufu binds to XG*i1 and X beta-catenin. In Xenopus embryos and mouse embryonic fibroblasts, Gli1 inhibits Wnt signaling under overexpression of beta-catenin, whereas beta-catenin stimulates Hh signaling under overexpression of Glil. Notably, endogenous Sufu is critically involved in this crosstalk The results suggest that XSufu may act as a common regulator of Hh and Wnt signaling and contribute to intertwining the two pathways. (C) 2011 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Suppressor-of-fused, Gli, beta-Catenin, Neural, Xenopus
in
Developmental Biology
volume
358
issue
1
pages
262 - 276
publisher
Elsevier
external identifiers
  • wos:000295198500025
  • pmid:21839734
  • scopus:80052627008
ISSN
1095-564X
DOI
10.1016/j.ydbio.2011.07.035
language
English
LU publication?
yes
id
8bc4dd6e-77d2-4d52-bb5d-52c8273a971e (old id 2179495)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21839734?dopt=Abstract
date added to LUP
2011-11-01 07:47:24
date last changed
2017-11-05 03:03:41
@article{8bc4dd6e-77d2-4d52-bb5d-52c8273a971e,
  abstract     = {Hedgehog (Hh) and Wnt proteins are important signals implicated in several aspects of embryonic development, including the early development of the central nervous system. We found that Xenopus Suppressor-of-fused (XSufu) affects neural induction and patterning by regulating the Hh/Gli and Wnt/beta-catenin pathways. Microinjection of XSufu mRNA induced expansion of the epidermis at the expense of neural plate tissue and caused enlargement of the eyes. An antisense morpholino oligonucleotide against XSufu had the opposite effect. Interestingly, both gain- and loss-of-function experiments resulted in a posterior shift of brain markers, suggesting a biphasic effect of XSufu on anteroposterior patterning. XSufu blocked early Wnt/beta-catenin signaling, as indicated by the suppression of XWnt8-induced secondary axis formation in mRNA-injected embryos, and activation of Wnt target genes in XSufu-MO-injected ectodermal explants. We show that XSufu binds to XG*i1 and X beta-catenin. In Xenopus embryos and mouse embryonic fibroblasts, Gli1 inhibits Wnt signaling under overexpression of beta-catenin, whereas beta-catenin stimulates Hh signaling under overexpression of Glil. Notably, endogenous Sufu is critically involved in this crosstalk The results suggest that XSufu may act as a common regulator of Hh and Wnt signaling and contribute to intertwining the two pathways. (C) 2011 Elsevier Inc. All rights reserved.},
  author       = {Tan Grahn, Hooi Min and Kriebel, Martin and Hou, Shirui and Pera, Edgar},
  issn         = {1095-564X},
  keyword      = {Suppressor-of-fused,Gli,beta-Catenin,Neural,Xenopus},
  language     = {eng},
  number       = {1},
  pages        = {262--276},
  publisher    = {Elsevier},
  series       = {Developmental Biology},
  title        = {The dual regulator Sufu integrates Hedgehog and Wnt signals in the early Xenopus embryo},
  url          = {http://dx.doi.org/10.1016/j.ydbio.2011.07.035},
  volume       = {358},
  year         = {2011},
}