No beneficial effect of two-layer storage compared with UW-storage on human islet isolation and transplantation
(2007) In Transplantation 84(7). p.864-869- Abstract
- Background. Shipment of pancreata between distant centers is frequently associated with prolonged cold ischemia time (CIT) that leads to poorer outcomes for islet transplantation. Clinical pilot trials have indicated that oxygenation of explanted human pancreata utilizing the two-layer method (TLM) allows the use of marginal donor pancreata for islet transplantation. The present study aimed to clarify whether TLM enhances the ischemic tolerance of human pancreata. Methods. We analyzed retrospectively the outcome of 200 human islet isolations performed after TLM preservation or storage in University of Wisconsin solution (UWS). Results. Donor characteristics and digestion parameters did not vary significantly between TLM-preserved and... (More)
- Background. Shipment of pancreata between distant centers is frequently associated with prolonged cold ischemia time (CIT) that leads to poorer outcomes for islet transplantation. Clinical pilot trials have indicated that oxygenation of explanted human pancreata utilizing the two-layer method (TLM) allows the use of marginal donor pancreata for islet transplantation. The present study aimed to clarify whether TLM enhances the ischemic tolerance of human pancreata. Methods. We analyzed retrospectively the outcome of 200 human islet isolations performed after TLM preservation or storage in University of Wisconsin solution (UWS). Results. Donor characteristics and digestion parameters did not vary significantly between TLM-preserved and UWSstored pancreata. No differences were observed between experimental groups with regard to islet yield, purity, or dynamic glucose stimulation index after either short or prolonged CIT. However, CIT and stimulation index were negatively correlated in each experimental group. The isolation outcome in donors aged ?60 years was not increased after TLM preservation when compared to UWS storage. No effect was observed regarding islet posttransplant function in recipients with established kidney grafts. Conclusions. The present study suggests that the ischemic tolerance of human pancreata cannot be extended by TLM preservation. In addition, TLM does not seem to improve the isolation outcome for pancreata from elderly donors. (Less)
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https://lup.lub.lu.se/record/654214
- author
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cold storage, two-layer method, clinical islet transplantation, marginal donors
- in
- Transplantation
- volume
- 84
- issue
- 7
- pages
- 864 - 869
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000250232600010
- scopus:38449089799
- ISSN
- 1534-6080
- DOI
- 10.1097/01.tp.0000284584.60600.ab
- language
- English
- LU publication?
- yes
- id
- 217c5c8c-09f1-4172-9b1d-0c0d57d5d56b (old id 654214)
- date added to LUP
- 2016-04-01 15:58:03
- date last changed
- 2022-04-15 01:11:54
@article{217c5c8c-09f1-4172-9b1d-0c0d57d5d56b, abstract = {{Background. Shipment of pancreata between distant centers is frequently associated with prolonged cold ischemia time (CIT) that leads to poorer outcomes for islet transplantation. Clinical pilot trials have indicated that oxygenation of explanted human pancreata utilizing the two-layer method (TLM) allows the use of marginal donor pancreata for islet transplantation. The present study aimed to clarify whether TLM enhances the ischemic tolerance of human pancreata. Methods. We analyzed retrospectively the outcome of 200 human islet isolations performed after TLM preservation or storage in University of Wisconsin solution (UWS). Results. Donor characteristics and digestion parameters did not vary significantly between TLM-preserved and UWSstored pancreata. No differences were observed between experimental groups with regard to islet yield, purity, or dynamic glucose stimulation index after either short or prolonged CIT. However, CIT and stimulation index were negatively correlated in each experimental group. The isolation outcome in donors aged ?60 years was not increased after TLM preservation when compared to UWS storage. No effect was observed regarding islet posttransplant function in recipients with established kidney grafts. Conclusions. The present study suggests that the ischemic tolerance of human pancreata cannot be extended by TLM preservation. In addition, TLM does not seem to improve the isolation outcome for pancreata from elderly donors.}}, author = {{Caballero-Corbalan, Jose and Eich, Torsten and Lundgren, Torbjoern and Foss, Aksel and Felldin, Marie and Källén, Ragnar and Salmela, Kalja and Tibell, Annika and Tufveson, Gunnar and Korsgren, Olle and Brandhorst, Daniel}}, issn = {{1534-6080}}, keywords = {{cold storage; two-layer method; clinical islet transplantation; marginal donors}}, language = {{eng}}, number = {{7}}, pages = {{864--869}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{Transplantation}}, title = {{No beneficial effect of two-layer storage compared with UW-storage on human islet isolation and transplantation}}, url = {{http://dx.doi.org/10.1097/01.tp.0000284584.60600.ab}}, doi = {{10.1097/01.tp.0000284584.60600.ab}}, volume = {{84}}, year = {{2007}}, }