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Preparation of hydrophilic poly(3-hydroxybutyrate) macroporous scaffolds through enzyme-mediated modifications

Guzmán, Daniel LU ; Kirsebom, Harald LU ; Solano, Carlos LU ; Quillaguaman, Jorge and Hatti-Kaul, Rajni LU (2011) In Journal of Bioactive and Compatible Polymers 26(5). p.452-463
Abstract
Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of... (More)
Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of human embryonic fibroblasts on the macroporous scaffolds confirmed that the cells proliferated and adhered to the materials. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biopolyester, poly(3-hydroxybutyrate), thermally induced phase, separation, lipase, glucosamine, gelatin, carbodiimide coupling
in
Journal of Bioactive and Compatible Polymers
volume
26
issue
5
pages
452 - 463
publisher
SAGE Publications Inc.
external identifiers
  • wos:000294816100002
  • scopus:80052733250
ISSN
0883-9115
DOI
10.1177/0883911511419970
language
English
LU publication?
yes
id
d78cabab-b395-4ead-9942-c98398ce08c4 (old id 2183487)
date added to LUP
2011-10-25 07:59:40
date last changed
2017-07-02 03:20:00
@article{d78cabab-b395-4ead-9942-c98398ce08c4,
  abstract     = {Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of human embryonic fibroblasts on the macroporous scaffolds confirmed that the cells proliferated and adhered to the materials.},
  author       = {Guzmán, Daniel and Kirsebom, Harald and Solano, Carlos and Quillaguaman, Jorge and Hatti-Kaul, Rajni},
  issn         = {0883-9115},
  keyword      = {biopolyester,poly(3-hydroxybutyrate),thermally induced phase,separation,lipase,glucosamine,gelatin,carbodiimide coupling},
  language     = {eng},
  number       = {5},
  pages        = {452--463},
  publisher    = {SAGE Publications Inc.},
  series       = {Journal of Bioactive and Compatible Polymers},
  title        = {Preparation of hydrophilic poly(3-hydroxybutyrate) macroporous scaffolds through enzyme-mediated modifications},
  url          = {http://dx.doi.org/10.1177/0883911511419970},
  volume       = {26},
  year         = {2011},
}