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Exogenously Administered Bombesin and Gastrin Releasing Peptide Contract the Female Rat Urethra In Vivo and In Vitro

Radziszewski, P.; Crayton, R.; Persson, Katarina LU ; Andersson, Karl-Erik LU and Mattiasson, Anders LU (2011) In Neurourology and Urodynamics 30(7). p.1388-1391
Abstract
Background: Bombesin (BOM) and gastrin releasing peptide (GRP) have been located to the lower urinary tract (LUT). However, there is a paucity of data demonstrating the impact of these endogenous peptides. Objectives: The aim of the present study was to investigate the contractile actions of BOM and GRP on the female rat urethra in vitro and in vivo. Female Sprague-Dawley rats (n = 37) weighing approximately 225 g were used. Intraurethral pressure was recorded by a catheter placed at the maximum pressure zone corresponding to the intrinsic urethral sphincter. Measurements: In vitro, changes in intraurethral pressure was conducted on perfused intact urethral/bladder preparations and are expressed as percentages of sphincteric intraurethral... (More)
Background: Bombesin (BOM) and gastrin releasing peptide (GRP) have been located to the lower urinary tract (LUT). However, there is a paucity of data demonstrating the impact of these endogenous peptides. Objectives: The aim of the present study was to investigate the contractile actions of BOM and GRP on the female rat urethra in vitro and in vivo. Female Sprague-Dawley rats (n = 37) weighing approximately 225 g were used. Intraurethral pressure was recorded by a catheter placed at the maximum pressure zone corresponding to the intrinsic urethral sphincter. Measurements: In vitro, changes in intraurethral pressure was conducted on perfused intact urethral/bladder preparations and are expressed as percentages of sphincteric intraurethral pressure achieved with noradrenaline. In vivo, changes in intraurethral pressure was conducted in anesthetized subjects and compared with the baseline intraurethral pressure and sham controls. Results: In vitro, the increase in intraurethral pressure induced by BOM was 23.6 +/- 3.2 cmH(2)O, exceeding the pressure evoked with NA by 9.6 cmH(2)O or 174.4% whereas GRP induced a maximum pressure of 10.7 +/- 1.6 cmH(2)O, an increase of 2.2 +/- 0.5 cmH(2)O or 82.9% (P < 0.05) of the NA evoked pressure. In vivo, the mean baseline pressure was 22.9 +/- 1.4 cmH(2)O. The intraurethral pressure evoked by BOM was 50.6 +/- 6.3 cmH(2)O (P < 0.05), and for GRP, the evoked intraurethral pressure was 56.2 +/- 13.4 cmH(2)O (P < 0.05). Conclusions: The present data suggest that both BOM and GRP may contribute to the control of continence by their contractile action on the sphincters of the LUT outflow region. Neurourol. Urodynam. 30:1388-1391, 2011. (C) 2011 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bombesin, gastrin releasing peptide, NANC, neuropeptides
in
Neurourology and Urodynamics
volume
30
issue
7
pages
1388 - 1391
publisher
John Wiley & Sons
external identifiers
  • wos:000294727800037
  • scopus:80052077293
ISSN
0733-2467
DOI
10.1002/nau.21056
language
English
LU publication?
yes
id
bc86898f-de18-4a01-b974-2484dd4b540e (old id 2186774)
date added to LUP
2011-11-01 07:46:39
date last changed
2017-11-05 03:14:37
@article{bc86898f-de18-4a01-b974-2484dd4b540e,
  abstract     = {Background: Bombesin (BOM) and gastrin releasing peptide (GRP) have been located to the lower urinary tract (LUT). However, there is a paucity of data demonstrating the impact of these endogenous peptides. Objectives: The aim of the present study was to investigate the contractile actions of BOM and GRP on the female rat urethra in vitro and in vivo. Female Sprague-Dawley rats (n = 37) weighing approximately 225 g were used. Intraurethral pressure was recorded by a catheter placed at the maximum pressure zone corresponding to the intrinsic urethral sphincter. Measurements: In vitro, changes in intraurethral pressure was conducted on perfused intact urethral/bladder preparations and are expressed as percentages of sphincteric intraurethral pressure achieved with noradrenaline. In vivo, changes in intraurethral pressure was conducted in anesthetized subjects and compared with the baseline intraurethral pressure and sham controls. Results: In vitro, the increase in intraurethral pressure induced by BOM was 23.6 +/- 3.2 cmH(2)O, exceeding the pressure evoked with NA by 9.6 cmH(2)O or 174.4% whereas GRP induced a maximum pressure of 10.7 +/- 1.6 cmH(2)O, an increase of 2.2 +/- 0.5 cmH(2)O or 82.9% (P &lt; 0.05) of the NA evoked pressure. In vivo, the mean baseline pressure was 22.9 +/- 1.4 cmH(2)O. The intraurethral pressure evoked by BOM was 50.6 +/- 6.3 cmH(2)O (P &lt; 0.05), and for GRP, the evoked intraurethral pressure was 56.2 +/- 13.4 cmH(2)O (P &lt; 0.05). Conclusions: The present data suggest that both BOM and GRP may contribute to the control of continence by their contractile action on the sphincters of the LUT outflow region. Neurourol. Urodynam. 30:1388-1391, 2011. (C) 2011 Wiley-Liss, Inc.},
  author       = {Radziszewski, P. and Crayton, R. and Persson, Katarina and Andersson, Karl-Erik and Mattiasson, Anders},
  issn         = {0733-2467},
  keyword      = {bombesin,gastrin releasing peptide,NANC,neuropeptides},
  language     = {eng},
  number       = {7},
  pages        = {1388--1391},
  publisher    = {John Wiley & Sons},
  series       = {Neurourology and Urodynamics},
  title        = {Exogenously Administered Bombesin and Gastrin Releasing Peptide Contract the Female Rat Urethra In Vivo and In Vitro},
  url          = {http://dx.doi.org/10.1002/nau.21056},
  volume       = {30},
  year         = {2011},
}