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A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus

Ljunggren, Mattias LU ; Willquist, Karin LU ; Zacchi, Guido LU and van Niel, Ed LU (2011) In Biotechnology for Biofuels 4:31(1). p.1-15
Abstract
Background: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2)producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H2 and osmotic pressure, as well as the liquid-to-gas mass transfer of H2.

Results: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were... (More)
Background: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2)producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H2 and osmotic pressure, as well as the liquid-to-gas mass transfer of H2.

Results: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were varied. The model was used to simulate the inhibition of growth

by H2 and solute concentrations, giving a critical dissolved H2 concentration of 2.2 mmol/L and an osmolarity of 0.27 to 29 mol/L. The inhibition by H2, being a function of the dissolved H2 concentration, was demonstrated to be mainly dependent on H2 productivity and mass transfer rate. The latter can be improved by increasing the stripping rate, thereby allowing higher H2 productivity. The experimentally determined degree of oversaturation of dissolved H2 was 12 to 34 times the equilibrium concentration and was comparable to the values given by the model.

Conclusions: The derived model is the first mechanistically based model for fermentative H2 production and provides useful information to improve the understanding of the growth behavior of C. saccharolyticus. The model

can be used to determine optimal operating conditions for H2 production regarding the substrate concentration and the stripping rate. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Caldicellulosiruptor saccharolyticus kinetic growth model hydrogen production osmolarity
in
Biotechnology for Biofuels
volume
4:31
issue
1
pages
1 - 15
publisher
BioMed Central
external identifiers
  • wos:000295872700001
  • scopus:80052665545
ISSN
1754-6834
DOI
10.1186/1754-6834-4-31
language
English
LU publication?
yes
id
b3380d0a-b6eb-4fc0-a015-8cb1ccf8cf73 (old id 2199498)
date added to LUP
2011-11-09 11:25:18
date last changed
2017-05-14 04:34:00
@article{b3380d0a-b6eb-4fc0-a015-8cb1ccf8cf73,
  abstract     = {Background: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2)producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H2 and osmotic pressure, as well as the liquid-to-gas mass transfer of H2.<br/><br>
Results: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were varied. The model was used to simulate the inhibition of growth<br/><br>
by H2 and solute concentrations, giving a critical dissolved H2 concentration of 2.2 mmol/L and an osmolarity of 0.27 to 29 mol/L. The inhibition by H2, being a function of the dissolved H2 concentration, was demonstrated to be mainly dependent on H2 productivity and mass transfer rate. The latter can be improved by increasing the stripping rate, thereby allowing higher H2 productivity. The experimentally determined degree of oversaturation of dissolved H2 was 12 to 34 times the equilibrium concentration and was comparable to the values given by the model.<br/><br>
Conclusions: The derived model is the first mechanistically based model for fermentative H2 production and provides useful information to improve the understanding of the growth behavior of C. saccharolyticus. The model<br/><br>
can be used to determine optimal operating conditions for H2 production regarding the substrate concentration and the stripping rate.},
  author       = {Ljunggren, Mattias and Willquist, Karin and Zacchi, Guido and van Niel, Ed},
  issn         = {1754-6834},
  keyword      = {Caldicellulosiruptor saccharolyticus kinetic growth model hydrogen production osmolarity},
  language     = {eng},
  number       = {1},
  pages        = {1--15},
  publisher    = {BioMed Central},
  series       = {Biotechnology for Biofuels},
  title        = {A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus},
  url          = {http://dx.doi.org/10.1186/1754-6834-4-31},
  volume       = {4:31},
  year         = {2011},
}