Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Touch signaling and thigmomorphogenesis are regulated by complementary CAMTA3- and JA-dependent pathways

Darwish, Essam LU ; Ghosh, Ritesh LU orcid ; Ontiveros-Cisneros, Abraham LU orcid ; Tran, Huy Cuong LU orcid ; Petersson, Marcus ; De Milde, Liesbeth ; Broda, Martyna ; Goossens, Alain ; Van Moerkercke, Alex LU and Khan, Kasim LU , et al. (2022) In Science Advances 8(20).
Abstract

Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators... (More)

Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators CAMTA1/2/3 are key regulators of JA-independent touch signaling. CAMTA1/2/3 cooperate to directly bind the promoters and activate gene expression of JA-independent touch marker genes like TCH2 and TCH4. In agreement, camta3 mutants show a near complete loss of thigmomorphogenesis and touch-induced delay of flowering. In conclusion, we have now identified key regulators of two independent touch-signaling pathways.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Science Advances
volume
8
issue
20
article number
eabm2091
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • pmid:35594358
  • scopus:85130613560
ISSN
2375-2548
DOI
10.1126/sciadv.abm2091
language
English
LU publication?
yes
additional info
Publisher Copyright: Copyright © 2022 The Authors,
id
21c16a10-e350-421b-9b67-cc97d27a92b7
date added to LUP
2022-08-22 10:53:36
date last changed
2024-06-13 18:21:47
@article{21c16a10-e350-421b-9b67-cc97d27a92b7,
  abstract     = {{<p>Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators CAMTA1/2/3 are key regulators of JA-independent touch signaling. CAMTA1/2/3 cooperate to directly bind the promoters and activate gene expression of JA-independent touch marker genes like TCH2 and TCH4. In agreement, camta3 mutants show a near complete loss of thigmomorphogenesis and touch-induced delay of flowering. In conclusion, we have now identified key regulators of two independent touch-signaling pathways.</p>}},
  author       = {{Darwish, Essam and Ghosh, Ritesh and Ontiveros-Cisneros, Abraham and Tran, Huy Cuong and Petersson, Marcus and De Milde, Liesbeth and Broda, Martyna and Goossens, Alain and Van Moerkercke, Alex and Khan, Kasim and Van Aken, Olivier}},
  issn         = {{2375-2548}},
  language     = {{eng}},
  number       = {{20}},
  publisher    = {{American Association for the Advancement of Science (AAAS)}},
  series       = {{Science Advances}},
  title        = {{Touch signaling and thigmomorphogenesis are regulated by complementary CAMTA3- and JA-dependent pathways}},
  url          = {{http://dx.doi.org/10.1126/sciadv.abm2091}},
  doi          = {{10.1126/sciadv.abm2091}},
  volume       = {{8}},
  year         = {{2022}},
}