Touch signaling and thigmomorphogenesis are regulated by complementary CAMTA3- and JA-dependent pathways
(2022) In Science Advances 8(20).- Abstract
Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators... (More)
Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators CAMTA1/2/3 are key regulators of JA-independent touch signaling. CAMTA1/2/3 cooperate to directly bind the promoters and activate gene expression of JA-independent touch marker genes like TCH2 and TCH4. In agreement, camta3 mutants show a near complete loss of thigmomorphogenesis and touch-induced delay of flowering. In conclusion, we have now identified key regulators of two independent touch-signaling pathways.
(Less)
- author
- organization
- publishing date
- 2022-05
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Science Advances
- volume
- 8
- issue
- 20
- article number
- eabm2091
- publisher
- American Association for the Advancement of Science (AAAS)
- external identifiers
-
- pmid:35594358
- scopus:85130613560
- ISSN
- 2375-2548
- DOI
- 10.1126/sciadv.abm2091
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: Copyright © 2022 The Authors,
- id
- 21c16a10-e350-421b-9b67-cc97d27a92b7
- date added to LUP
- 2022-08-22 10:53:36
- date last changed
- 2024-09-20 03:30:24
@article{21c16a10-e350-421b-9b67-cc97d27a92b7, abstract = {{<p>Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators CAMTA1/2/3 are key regulators of JA-independent touch signaling. CAMTA1/2/3 cooperate to directly bind the promoters and activate gene expression of JA-independent touch marker genes like TCH2 and TCH4. In agreement, camta3 mutants show a near complete loss of thigmomorphogenesis and touch-induced delay of flowering. In conclusion, we have now identified key regulators of two independent touch-signaling pathways.</p>}}, author = {{Darwish, Essam and Ghosh, Ritesh and Ontiveros-Cisneros, Abraham and Tran, Huy Cuong and Petersson, Marcus and De Milde, Liesbeth and Broda, Martyna and Goossens, Alain and Van Moerkercke, Alex and Khan, Kasim and Van Aken, Olivier}}, issn = {{2375-2548}}, language = {{eng}}, number = {{20}}, publisher = {{American Association for the Advancement of Science (AAAS)}}, series = {{Science Advances}}, title = {{Touch signaling and thigmomorphogenesis are regulated by complementary CAMTA3- and JA-dependent pathways}}, url = {{http://dx.doi.org/10.1126/sciadv.abm2091}}, doi = {{10.1126/sciadv.abm2091}}, volume = {{8}}, year = {{2022}}, }