Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Response to dietary l-glutamine supplementation in weaned piglets : A serum metabolomic comparison and hepatic metabolic regulation analysis

Xiao, Y. P. ; Wu, T. X. ; Sun, J. M. LU orcid ; Yang, L. ; Hong, Q. H. ; Chen, A. G. and Yang, C. M. (2012) In Journal of Animal Science 90(12). p.4421-4430
Abstract

A novel metabolomic method based on gas chromatography-mass spectrometry was applied to investigate serum metabolites in response to dietary Gln supplementation in piglets. Sixteen, 21-d-old pigs were weaned and assigned randomly to 2 isonitrogenous diets: 1) Gln diet, which contained 1% L-Gln (as-fed basis), and 2) control diet, which contained LAla to make this diet isonitrogenous with the Gln diet. Serum samples were collected to characterize metabolites after a 30-d treatment. In addition, 4 liver samples per treatment were collected to examine enzyme activity and gene expression involved in metabolic regulation. Results indicated that 12 metabolites were altered (P < 0.05) by Gln treatment, including carbohydrates, AA, and fatty... (More)

A novel metabolomic method based on gas chromatography-mass spectrometry was applied to investigate serum metabolites in response to dietary Gln supplementation in piglets. Sixteen, 21-d-old pigs were weaned and assigned randomly to 2 isonitrogenous diets: 1) Gln diet, which contained 1% L-Gln (as-fed basis), and 2) control diet, which contained LAla to make this diet isonitrogenous with the Gln diet. Serum samples were collected to characterize metabolites after a 30-d treatment. In addition, 4 liver samples per treatment were collected to examine enzyme activity and gene expression involved in metabolic regulation. Results indicated that 12 metabolites were altered (P < 0.05) by Gln treatment, including carbohydrates, AA, and fatty acids. A leave-one-out cross validation of random forest analysis indicated that Pro was most important among the 12 metabolites. Thus, these data demonstrate that the control and Gln-supplemented pigs differed (P < 0.05) in terms of metabolism of carbohydrates, Pro, Tyr, and glycerophospholipids. Principal component analysis yielded separate clusters of profi les between the Gln and control groups. Metabolic enzyme activities of Ala aminotransferase and hexokinase increased by 26.8% (P = 0.026) and 26.2% (P = 0.004) in the liver of Gln-supplemented pigs vs. control, respectively, whereas pyruvate kinase (PK) activity decreased by 29.1% (P = 0.001). The gene expression of PK in the liver decreased by 66.1% (P = 0.034) by Gln treatment for 30 d. No differences were observed for the mRNA abundance of mammalian target of rapamycin and PPARγ. On the basis of these data, Gln treatment affected carbohydrate, lipid, and AA metabolism in the whole body of the early weaned piglets. These fi ndings provide insight into specifi c metabolic pathways and lay the groundwork for the complex metabolic alteration in response to dietary Gln supplementation of pigs.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Glutamine, Liver, Metabolic regulation, Metabolomics, Piglet, Serum
in
Journal of Animal Science
volume
90
issue
12
pages
4421 - 4430
publisher
Oxford University Press
external identifiers
  • pmid:23255817
  • scopus:84882604964
ISSN
0021-8812
DOI
10.2527/jas.2012-5039
language
English
LU publication?
no
id
21cfec93-e807-4df5-85d7-2f14f114165f
date added to LUP
2023-05-03 22:09:51
date last changed
2024-03-08 00:35:55
@article{21cfec93-e807-4df5-85d7-2f14f114165f,
  abstract     = {{<p>A novel metabolomic method based on gas chromatography-mass spectrometry was applied to investigate serum metabolites in response to dietary Gln supplementation in piglets. Sixteen, 21-d-old pigs were weaned and assigned randomly to 2 isonitrogenous diets: 1) Gln diet, which contained 1% L-Gln (as-fed basis), and 2) control diet, which contained LAla to make this diet isonitrogenous with the Gln diet. Serum samples were collected to characterize metabolites after a 30-d treatment. In addition, 4 liver samples per treatment were collected to examine enzyme activity and gene expression involved in metabolic regulation. Results indicated that 12 metabolites were altered (P &lt; 0.05) by Gln treatment, including carbohydrates, AA, and fatty acids. A leave-one-out cross validation of random forest analysis indicated that Pro was most important among the 12 metabolites. Thus, these data demonstrate that the control and Gln-supplemented pigs differed (P &lt; 0.05) in terms of metabolism of carbohydrates, Pro, Tyr, and glycerophospholipids. Principal component analysis yielded separate clusters of profi les between the Gln and control groups. Metabolic enzyme activities of Ala aminotransferase and hexokinase increased by 26.8% (P = 0.026) and 26.2% (P = 0.004) in the liver of Gln-supplemented pigs vs. control, respectively, whereas pyruvate kinase (PK) activity decreased by 29.1% (P = 0.001). The gene expression of PK in the liver decreased by 66.1% (P = 0.034) by Gln treatment for 30 d. No differences were observed for the mRNA abundance of mammalian target of rapamycin and PPARγ. On the basis of these data, Gln treatment affected carbohydrate, lipid, and AA metabolism in the whole body of the early weaned piglets. These fi ndings provide insight into specifi c metabolic pathways and lay the groundwork for the complex metabolic alteration in response to dietary Gln supplementation of pigs.</p>}},
  author       = {{Xiao, Y. P. and Wu, T. X. and Sun, J. M. and Yang, L. and Hong, Q. H. and Chen, A. G. and Yang, C. M.}},
  issn         = {{0021-8812}},
  keywords     = {{Glutamine; Liver; Metabolic regulation; Metabolomics; Piglet; Serum}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{4421--4430}},
  publisher    = {{Oxford University Press}},
  series       = {{Journal of Animal Science}},
  title        = {{Response to dietary l-glutamine supplementation in weaned piglets : A serum metabolomic comparison and hepatic metabolic regulation analysis}},
  url          = {{http://dx.doi.org/10.2527/jas.2012-5039}},
  doi          = {{10.2527/jas.2012-5039}},
  volume       = {{90}},
  year         = {{2012}},
}