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Use of Lung Weight as Biomarker for Assessment of Lung Toxicity in Rat Inhalation Studies

Wahlstrom, Erik; Ollerstam, Anna; Sundius, Lena LU and Zhang, Hui (2013) In Toxicologic Pathology 41(6). p.902-912
Abstract
Subacute inhalation study (1 week or 2 weeks) is an important process for screening out inhaled compounds causing lung irritation. To investigate whether the lung weight can be used as an indicator for acute lung injury, we have analyzed retrospectively the lung weight data from 30 studies in rats exposed to dry powder inhalation. The lung weight change was correlated with lung histopathology in the majority of studies (25 of 30), showing as either both changed or both unchanged. The sensitivity and specificity of using the weight change in lungs as biomarker for predicting lung histopathology in these studies were over 80%. The pattern of lung weight change was often similar in the 1- to 2- week studies and the 4-week studies. Our... (More)
Subacute inhalation study (1 week or 2 weeks) is an important process for screening out inhaled compounds causing lung irritation. To investigate whether the lung weight can be used as an indicator for acute lung injury, we have analyzed retrospectively the lung weight data from 30 studies in rats exposed to dry powder inhalation. The lung weight change was correlated with lung histopathology in the majority of studies (25 of 30), showing as either both changed or both unchanged. The sensitivity and specificity of using the weight change in lungs as biomarker for predicting lung histopathology in these studies were over 80%. The pattern of lung weight change was often similar in the 1- to 2- week studies and the 4-week studies. Our analysis of covariance model showed that a study with 40 rats (5 males + 5 females/group and 4 groups) could detect lung weight change greater than 10% to 20% of control value. These results suggested that lung weight is a useful indicator for identifying acute lung toxic effect by inhaled compounds in these subacute inhalation studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Inhalation, lung weight, lung histopathology, toxicology, statistical, analysis, biomarkers, rat
in
Toxicologic Pathology
volume
41
issue
6
pages
902 - 912
publisher
SAGE Publications Inc.
external identifiers
  • wos:000321898400009
  • scopus:84880408538
ISSN
0192-6233
DOI
10.1177/0192623312470763
language
English
LU publication?
yes
id
21d4dbd0-43f1-499c-a3de-96abf1e0477d (old id 3975709)
date added to LUP
2013-09-02 07:35:42
date last changed
2019-02-20 07:15:52
@article{21d4dbd0-43f1-499c-a3de-96abf1e0477d,
  abstract     = {Subacute inhalation study (1 week or 2 weeks) is an important process for screening out inhaled compounds causing lung irritation. To investigate whether the lung weight can be used as an indicator for acute lung injury, we have analyzed retrospectively the lung weight data from 30 studies in rats exposed to dry powder inhalation. The lung weight change was correlated with lung histopathology in the majority of studies (25 of 30), showing as either both changed or both unchanged. The sensitivity and specificity of using the weight change in lungs as biomarker for predicting lung histopathology in these studies were over 80%. The pattern of lung weight change was often similar in the 1- to 2- week studies and the 4-week studies. Our analysis of covariance model showed that a study with 40 rats (5 males + 5 females/group and 4 groups) could detect lung weight change greater than 10% to 20% of control value. These results suggested that lung weight is a useful indicator for identifying acute lung toxic effect by inhaled compounds in these subacute inhalation studies.},
  author       = {Wahlstrom, Erik and Ollerstam, Anna and Sundius, Lena and Zhang, Hui},
  issn         = {0192-6233},
  keyword      = {Inhalation,lung weight,lung histopathology,toxicology,statistical,analysis,biomarkers,rat},
  language     = {eng},
  number       = {6},
  pages        = {902--912},
  publisher    = {SAGE Publications Inc.},
  series       = {Toxicologic Pathology},
  title        = {Use of Lung Weight as Biomarker for Assessment of Lung Toxicity in Rat Inhalation Studies},
  url          = {http://dx.doi.org/10.1177/0192623312470763},
  volume       = {41},
  year         = {2013},
}