The Native Reaction Centre of Photosystem II: A New Paradigm for P680
(2004) In Australian Journal of Chemistry 57(12). p.1179-1183- Abstract
- Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that... (More)
- Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that in CP43 can be distinguished in the hole-burning action spectrum and high-resolution hole-burning spectra. An important observation is that 685–700 nm illumination gives rise to efficient P680 charge separation, as established by QA− formation. This leads to a new paradigm for P680. The charge-separating state has surprisingly weak absorption and extends to 700 nm. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/141363
- author
- Hughes, Joseph L ; Prince, Barry J ; Peterson Årsköld, Sindra LU ; Smith, Paul J ; Pace, Ron J ; Riesen, Hans and Krausz, Elmars
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Australian Journal of Chemistry
- volume
- 57
- issue
- 12
- pages
- 1179 - 1183
- publisher
- CSIRO Publishing
- external identifiers
-
- wos:000225599500014
- scopus:11144253679
- ISSN
- 0004-9425
- DOI
- 10.1071/CH04140
- language
- English
- LU publication?
- yes
- id
- 21e41b01-f55f-4531-8422-249447de1101 (old id 141363)
- date added to LUP
- 2016-04-01 15:53:47
- date last changed
- 2022-01-28 07:49:41
@article{21e41b01-f55f-4531-8422-249447de1101, abstract = {{Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that in CP43 can be distinguished in the hole-burning action spectrum and high-resolution hole-burning spectra. An important observation is that 685–700 nm illumination gives rise to efficient P680 charge separation, as established by QA− formation. This leads to a new paradigm for P680. The charge-separating state has surprisingly weak absorption and extends to 700 nm.}}, author = {{Hughes, Joseph L and Prince, Barry J and Peterson Årsköld, Sindra and Smith, Paul J and Pace, Ron J and Riesen, Hans and Krausz, Elmars}}, issn = {{0004-9425}}, language = {{eng}}, number = {{12}}, pages = {{1179--1183}}, publisher = {{CSIRO Publishing}}, series = {{Australian Journal of Chemistry}}, title = {{The Native Reaction Centre of Photosystem II: A New Paradigm for P680}}, url = {{http://dx.doi.org/10.1071/CH04140}}, doi = {{10.1071/CH04140}}, volume = {{57}}, year = {{2004}}, }