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Adsorption of HSA, IgG and laminin-1 on model titania surfaces - effects of glow discharge treatment on competitively adsorbed film composition.

Santos, Olga LU ; Svendsen, Ida E; Lindh, Liselott and Arnebrant, Thomas (2011) In Biofouling 27(9). p.1003-1015
Abstract
This study investigated the effect of glow discharge treatment of titania surfaces on plasma protein adsorption, by means of ellipsometry and mechanically assisted SDS elution. The adsorption and film elution of three plasma proteins, viz. human serum albumin (HSA), human immunoglobulin G (IgG) and laminin-1, as well as competitive adsorption from a mixture of the three proteins, showed that the adsorbed amount of the individual proteins after 1 h increased in the order HSA <IgG <laminin-1 ≤ protein mixture. Film elutability showed that 30 min of SDS interaction resulted in almost complete removal of adsorbed films. No difference in the total adsorbed amounts of individual proteins, or from the mixture, was observed between untreated... (More)
This study investigated the effect of glow discharge treatment of titania surfaces on plasma protein adsorption, by means of ellipsometry and mechanically assisted SDS elution. The adsorption and film elution of three plasma proteins, viz. human serum albumin (HSA), human immunoglobulin G (IgG) and laminin-1, as well as competitive adsorption from a mixture of the three proteins, showed that the adsorbed amount of the individual proteins after 1 h increased in the order HSA <IgG <laminin-1 ≤ protein mixture. Film elutability showed that 30 min of SDS interaction resulted in almost complete removal of adsorbed films. No difference in the total adsorbed amounts of individual proteins, or from the mixture, was observed between untreated and glow discharge treated titania surfaces. However, the composition of the adsorbed films from the mixture differed between the untreated and glow discharge treated substrata. On glow discharge-treated titania the fraction of HSA increased, the fraction of laminin-1 decreased and the fraction of IgG was unchanged compared to the adsorption on the untreated titania, which was attributed to protein-protein interactions and competitive/associative adsorption behaviour. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biofouling
volume
27
issue
9
pages
1003 - 1015
publisher
Taylor & Francis
external identifiers
  • pmid:22004177
  • scopus:84857071548
ISSN
1029-2454
DOI
10.1080/08927014.2011.622440
language
English
LU publication?
yes
id
fbcd1ebc-001f-4e79-8135-e59c3fa10116 (old id 2200442)
date added to LUP
2011-11-09 12:17:29
date last changed
2017-01-01 06:26:48
@article{fbcd1ebc-001f-4e79-8135-e59c3fa10116,
  abstract     = {This study investigated the effect of glow discharge treatment of titania surfaces on plasma protein adsorption, by means of ellipsometry and mechanically assisted SDS elution. The adsorption and film elution of three plasma proteins, viz. human serum albumin (HSA), human immunoglobulin G (IgG) and laminin-1, as well as competitive adsorption from a mixture of the three proteins, showed that the adsorbed amount of the individual proteins after 1 h increased in the order HSA &lt;IgG &lt;laminin-1 ≤ protein mixture. Film elutability showed that 30 min of SDS interaction resulted in almost complete removal of adsorbed films. No difference in the total adsorbed amounts of individual proteins, or from the mixture, was observed between untreated and glow discharge treated titania surfaces. However, the composition of the adsorbed films from the mixture differed between the untreated and glow discharge treated substrata. On glow discharge-treated titania the fraction of HSA increased, the fraction of laminin-1 decreased and the fraction of IgG was unchanged compared to the adsorption on the untreated titania, which was attributed to protein-protein interactions and competitive/associative adsorption behaviour.},
  author       = {Santos, Olga and Svendsen, Ida E and Lindh, Liselott and Arnebrant, Thomas},
  issn         = {1029-2454},
  language     = {eng},
  number       = {9},
  pages        = {1003--1015},
  publisher    = {Taylor & Francis},
  series       = {Biofouling},
  title        = {Adsorption of HSA, IgG and laminin-1 on model titania surfaces - effects of glow discharge treatment on competitively adsorbed film composition.},
  url          = {http://dx.doi.org/10.1080/08927014.2011.622440},
  volume       = {27},
  year         = {2011},
}