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Serine/arginine-rich protein 30c activates human papillomavirus type 16 L1 mRNA expression via a bimodal mechanism

Somberg, Monika; Li, Xiaoze LU ; Johansson, Cecilia; Orru, Beatrice; Chang, Roger; Rush, Margaret; Fay, Joanna; Ryan, Fergus and Schwartz, Stefan LU (2011) In Journal of General Virology 92. p.2411-2421
Abstract
Two splice sites on the human papillomavirus type 16 (HPV-16) genome are used exclusively by the late capsid protein L1 mRNAs: SD3632 and SA5639. These splice sites are suppressed in mitotic cells. This study showed that serine/arginine-rich protein 30c (SRp30c), also named SFRS9, activated both SD3632 and SA5639 and induced production of L1 mRNA. Activation of HPV-16 L1 mRNA splicing by SRp30c required an intact arginine/serine-repeat (RS) domain of SRp30c. In addition to this effect, SRp30c could enhance L1 mRNA production indirectly by inhibiting the early 3'-splice site SA3358, which competed with the late 3'-splice site SA5639. SRp30c bound directly to sequences downstream of SA3358, suggesting that SRp30c inhibited the enhancer at... (More)
Two splice sites on the human papillomavirus type 16 (HPV-16) genome are used exclusively by the late capsid protein L1 mRNAs: SD3632 and SA5639. These splice sites are suppressed in mitotic cells. This study showed that serine/arginine-rich protein 30c (SRp30c), also named SFRS9, activated both SD3632 and SA5639 and induced production of L1 mRNA. Activation of HPV-16 L1 mRNA splicing by SRp30c required an intact arginine/serine-repeat (RS) domain of SRp30c. In addition to this effect, SRp30c could enhance L1 mRNA production indirectly by inhibiting the early 3'-splice site SA3358, which competed with the late 3'-splice site SA5639. SRp30c bound directly to sequences downstream of SA3358, suggesting that SRp30c inhibited the enhancer at SA3358 and caused a redirection of splicing to the late 3'-splice site SA5639. This inhibitory effect of SRp30c was independent of its RS domain. These results suggest that SRp30c can activate HPV-16 L1 mRNA expression via a bimodal mechanism: directly by stimulating splicing to late splice sites and indirectly by inhibiting competing early splice sites. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
92
pages
2411 - 2421
publisher
Society for General Microbiology
external identifiers
  • wos:000295856000022
  • scopus:80052922847
ISSN
1465-2099
DOI
10.1099/vir.0.033183-0
language
English
LU publication?
yes
id
cda6a9d8-ce28-462f-b760-0198be333fc1 (old id 2208232)
date added to LUP
2011-12-01 08:28:16
date last changed
2017-10-22 04:13:15
@article{cda6a9d8-ce28-462f-b760-0198be333fc1,
  abstract     = {Two splice sites on the human papillomavirus type 16 (HPV-16) genome are used exclusively by the late capsid protein L1 mRNAs: SD3632 and SA5639. These splice sites are suppressed in mitotic cells. This study showed that serine/arginine-rich protein 30c (SRp30c), also named SFRS9, activated both SD3632 and SA5639 and induced production of L1 mRNA. Activation of HPV-16 L1 mRNA splicing by SRp30c required an intact arginine/serine-repeat (RS) domain of SRp30c. In addition to this effect, SRp30c could enhance L1 mRNA production indirectly by inhibiting the early 3'-splice site SA3358, which competed with the late 3'-splice site SA5639. SRp30c bound directly to sequences downstream of SA3358, suggesting that SRp30c inhibited the enhancer at SA3358 and caused a redirection of splicing to the late 3'-splice site SA5639. This inhibitory effect of SRp30c was independent of its RS domain. These results suggest that SRp30c can activate HPV-16 L1 mRNA expression via a bimodal mechanism: directly by stimulating splicing to late splice sites and indirectly by inhibiting competing early splice sites.},
  author       = {Somberg, Monika and Li, Xiaoze and Johansson, Cecilia and Orru, Beatrice and Chang, Roger and Rush, Margaret and Fay, Joanna and Ryan, Fergus and Schwartz, Stefan},
  issn         = {1465-2099},
  language     = {eng},
  pages        = {2411--2421},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {Serine/arginine-rich protein 30c activates human papillomavirus type 16 L1 mRNA expression via a bimodal mechanism},
  url          = {http://dx.doi.org/10.1099/vir.0.033183-0},
  volume       = {92},
  year         = {2011},
}