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Bacterial pollution induced community tolerance (PICT) to Cu and interactions with pH in long-term polluted vineyard soils

Fernandez-Calvino, David; Arias-Estevez, Manuel; Diaz-Ravina, Montserrat and Bååth, Erland LU (2011) In Soil Biology & Biochemistry 43(11). p.2324-2331
Abstract
Pollution induced community tolerance (PICT) has been suggested as an end-point measurement less affected by confounding environmental factors compared to standard methods of microbial growth, activity and community composition. We evaluated the use of PICT to determine Cu toxicity in vineyard soils polluted with Cu based fungicides (25-1120 mg Cu kg(-1)). These soils also varied in pH (4.3-7.3), organic C (0.31-6.91%) and texture (14-56% silt). PICT was estimated as bacterial community tolerance to Cu measured by the [H-3]leucine incorporation method. Bacterial tolerance to Cu increased 9 times in the most polluted compared to the unpolluted soils. Cu tolerance was also affected to a minor degree by pH, organic C and soil texture. Lower... (More)
Pollution induced community tolerance (PICT) has been suggested as an end-point measurement less affected by confounding environmental factors compared to standard methods of microbial growth, activity and community composition. We evaluated the use of PICT to determine Cu toxicity in vineyard soils polluted with Cu based fungicides (25-1120 mg Cu kg(-1)). These soils also varied in pH (4.3-7.3), organic C (0.31-6.91%) and texture (14-56% silt). PICT was estimated as bacterial community tolerance to Cu measured by the [H-3]leucine incorporation method. Bacterial tolerance to Cu increased 9 times in the most polluted compared to the unpolluted soils. Cu tolerance was also affected to a minor degree by pH, organic C and soil texture. Lower bacterial tolerance was found in soils with high pH and organic C, probably due to Cu becoming less bioavailable in soils with high pH and organic C content. The silt content appeared to increase bacterial tolerance, probably due to fine soil particles decreasing Cu bioavailability during the PICT detection phase. Despite the effects of other environmental factors, the main determinant of increased bacterial community tolerance to Cu was the pollution level. PICT measured with the leucine incorporation technique thus appears to be a sensitive and stable concept to evaluate toxic impacts, unless soils with very different pH, organic C or texture are studied. (C) 2011 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cu pollution, PICT, Bacterial growth, Fungal growth
in
Soil Biology & Biochemistry
volume
43
issue
11
pages
2324 - 2331
publisher
Elsevier
external identifiers
  • wos:000295745700015
  • scopus:80052216485
ISSN
0038-0717
DOI
10.1016/j.soilbio.2011.08.001
language
English
LU publication?
yes
id
09a2e7d8-04b4-44b4-9cc4-35a82d0c3dcb (old id 2211624)
date added to LUP
2011-11-24 13:32:24
date last changed
2017-08-27 04:59:06
@article{09a2e7d8-04b4-44b4-9cc4-35a82d0c3dcb,
  abstract     = {Pollution induced community tolerance (PICT) has been suggested as an end-point measurement less affected by confounding environmental factors compared to standard methods of microbial growth, activity and community composition. We evaluated the use of PICT to determine Cu toxicity in vineyard soils polluted with Cu based fungicides (25-1120 mg Cu kg(-1)). These soils also varied in pH (4.3-7.3), organic C (0.31-6.91%) and texture (14-56% silt). PICT was estimated as bacterial community tolerance to Cu measured by the [H-3]leucine incorporation method. Bacterial tolerance to Cu increased 9 times in the most polluted compared to the unpolluted soils. Cu tolerance was also affected to a minor degree by pH, organic C and soil texture. Lower bacterial tolerance was found in soils with high pH and organic C, probably due to Cu becoming less bioavailable in soils with high pH and organic C content. The silt content appeared to increase bacterial tolerance, probably due to fine soil particles decreasing Cu bioavailability during the PICT detection phase. Despite the effects of other environmental factors, the main determinant of increased bacterial community tolerance to Cu was the pollution level. PICT measured with the leucine incorporation technique thus appears to be a sensitive and stable concept to evaluate toxic impacts, unless soils with very different pH, organic C or texture are studied. (C) 2011 Elsevier Ltd. All rights reserved.},
  author       = {Fernandez-Calvino, David and Arias-Estevez, Manuel and Diaz-Ravina, Montserrat and Bååth, Erland},
  issn         = {0038-0717},
  keyword      = {Cu pollution,PICT,Bacterial growth,Fungal growth},
  language     = {eng},
  number       = {11},
  pages        = {2324--2331},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Bacterial pollution induced community tolerance (PICT) to Cu and interactions with pH in long-term polluted vineyard soils},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2011.08.001},
  volume       = {43},
  year         = {2011},
}