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WUSCHEL protein movement mediates stem cell homeostasis in the Arabidopsis shoot apex

Yadav, Ram Kishor; Perales, Mariano; Gruel, Jeremy; Girke, Thomas; Jönsson, Henrik LU and Reddy, G. Venugopala (2011) In Genes & Development 25(19). p.2025-2030
Abstract
WUSCHEL (WUS) is a homeodomain transcription factor produced in cells of the niche/organizing center (OC) of shoot apical meristems. WUS specifies stem cell fate and also restricts its own levels by activating a negative regulator, CLAVATA3 (CLV3), in adjacent cells of the central zone (CZ). Here we show that the WUS protein, after being synthesized in cells of the OC, migrates into the CZ, where it activates CLV3 transcription by binding to its promoter elements. Using a computational model, we show that maintenance of the WUS gradient is essential to regulate stem cell number. Migration of a stem cell-inducing transcription factor into adjacent cells to activate a negative regulator, thereby restricting its own accumulation, is a theme... (More)
WUSCHEL (WUS) is a homeodomain transcription factor produced in cells of the niche/organizing center (OC) of shoot apical meristems. WUS specifies stem cell fate and also restricts its own levels by activating a negative regulator, CLAVATA3 (CLV3), in adjacent cells of the central zone (CZ). Here we show that the WUS protein, after being synthesized in cells of the OC, migrates into the CZ, where it activates CLV3 transcription by binding to its promoter elements. Using a computational model, we show that maintenance of the WUS gradient is essential to regulate stem cell number. Migration of a stem cell-inducing transcription factor into adjacent cells to activate a negative regulator, thereby restricting its own accumulation, is a theme that is unique to plant stem cell niches. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CLAVATA3, shoot apical meristem, CLAVATA1, central zone, niche
in
Genes & Development
volume
25
issue
19
pages
2025 - 2030
publisher
Cold Spring Harbor Laboratory Press
external identifiers
  • wos:000295775600003
  • scopus:80053629973
ISSN
1549-5477
DOI
10.1101/gad.17258511
language
English
LU publication?
yes
id
ee56ecfa-5527-40b6-86b1-bfb3434d5998 (old id 2212790)
date added to LUP
2011-11-23 13:34:56
date last changed
2017-10-22 04:20:25
@article{ee56ecfa-5527-40b6-86b1-bfb3434d5998,
  abstract     = {WUSCHEL (WUS) is a homeodomain transcription factor produced in cells of the niche/organizing center (OC) of shoot apical meristems. WUS specifies stem cell fate and also restricts its own levels by activating a negative regulator, CLAVATA3 (CLV3), in adjacent cells of the central zone (CZ). Here we show that the WUS protein, after being synthesized in cells of the OC, migrates into the CZ, where it activates CLV3 transcription by binding to its promoter elements. Using a computational model, we show that maintenance of the WUS gradient is essential to regulate stem cell number. Migration of a stem cell-inducing transcription factor into adjacent cells to activate a negative regulator, thereby restricting its own accumulation, is a theme that is unique to plant stem cell niches.},
  author       = {Yadav, Ram Kishor and Perales, Mariano and Gruel, Jeremy and Girke, Thomas and Jönsson, Henrik and Reddy, G. Venugopala},
  issn         = {1549-5477},
  keyword      = {CLAVATA3,shoot apical meristem,CLAVATA1,central zone,niche},
  language     = {eng},
  number       = {19},
  pages        = {2025--2030},
  publisher    = {Cold Spring Harbor Laboratory Press},
  series       = {Genes & Development},
  title        = {WUSCHEL protein movement mediates stem cell homeostasis in the Arabidopsis shoot apex},
  url          = {http://dx.doi.org/10.1101/gad.17258511},
  volume       = {25},
  year         = {2011},
}