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Capacitive immunosensor for the detection of host cell proteins.

Teeparuksapun, Kosin LU ; Hedström, Martin LU ; Kanatharana, Proespichaya; Thavarungkul, Panote and Mattiasson, Bo LU (2012) In Journal of Biotechnology 157. p.207-213
Abstract
A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby... (More)
A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing negative influence from non-specific binding to the sensor surface. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
157
pages
207 - 213
publisher
Elsevier
external identifiers
  • wos:000298635200029
  • pmid:22100266
  • scopus:84855220149
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2011.11.004
language
English
LU publication?
yes
id
07875e43-18fd-4f98-91dc-7e3bdb7996d9 (old id 2220600)
date added to LUP
2011-12-13 17:16:22
date last changed
2017-11-12 03:07:04
@article{07875e43-18fd-4f98-91dc-7e3bdb7996d9,
  abstract     = {A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a mode system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing negative influence from non-specific binding to the sensor surface.},
  author       = {Teeparuksapun, Kosin and Hedström, Martin and Kanatharana, Proespichaya and Thavarungkul, Panote and Mattiasson, Bo},
  issn         = {1873-4863},
  language     = {eng},
  pages        = {207--213},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Capacitive immunosensor for the detection of host cell proteins.},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2011.11.004},
  volume       = {157},
  year         = {2012},
}