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Emergence of NK cell progenitors and functionally competent NK cell lineage subsets in the early mouse embryo.

Tang, Yanjuan; Peitzsch, Claudia; NozadCharoudeh, Hojjatollah LU ; Cheng, Min LU ; Chaves Guerrero, Patricia LU ; Jacobsen, Sten Eirik W LU and Sitnicka Quinn, Ewa LU (2012) In Blood 120(1). p.63-75
Abstract
The earliest stages of natural killer (NK) cell development are not well characterized. In this study, we investigated in different fetal hematopoietic tissues how NK cell progenitors and their mature NK cell progeny emerge and expand during fetal development. Here we demonstrate, for the first time, that the counterpart of adult bone marrow Lin(-)CD122(+)NK1.1(-)DX5(-) NK cell progenitor (NKP) emerges in the fetal liver at embryonic day (E) 13.5. Following NKP expansion, immature NK cells emerge at day E14.5 in the liver and E15.5 in the spleen. Thymic NK cells arise at day E15.5, while functionally competent cytotoxic NK cells were present in the liver and spleen at day E16.5 and E17.5, respectively. Fetal NKPs failed to produce B and... (More)
The earliest stages of natural killer (NK) cell development are not well characterized. In this study, we investigated in different fetal hematopoietic tissues how NK cell progenitors and their mature NK cell progeny emerge and expand during fetal development. Here we demonstrate, for the first time, that the counterpart of adult bone marrow Lin(-)CD122(+)NK1.1(-)DX5(-) NK cell progenitor (NKP) emerges in the fetal liver at embryonic day (E) 13.5. Following NKP expansion, immature NK cells emerge at day E14.5 in the liver and E15.5 in the spleen. Thymic NK cells arise at day E15.5, while functionally competent cytotoxic NK cells were present in the liver and spleen at day E16.5 and E17.5, respectively. Fetal NKPs failed to produce B and myeloid cells, but sustained combined NK and T lineage potential at the single cell level. NKPs were also found in the fetal blood, spleen and thymus. These findings demonstrate the emergence and expansion of bipotent NK/T cell progenitor during fetal and adult lymphopoiesis, further supporting that NK/T lineage restriction is taking place prethymically. Uncovering the earliest NK cell developmental stages will provide important clues helping to understand the origin of diverse NK cell subsets, their progenitors and key regulators. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
120
issue
1
pages
63 - 75
publisher
American Society of Hematology
external identifiers
  • wos:000307411100014
  • pmid:22072559
  • scopus:84863599852
ISSN
1528-0020
DOI
10.1182/blood-2011-02-337980
language
English
LU publication?
yes
id
6ce8d310-64c9-4c80-8f81-e2da49017b52 (old id 2220969)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22072559?dopt=Abstract
date added to LUP
2011-12-02 20:17:01
date last changed
2017-01-12 08:44:04
@article{6ce8d310-64c9-4c80-8f81-e2da49017b52,
  abstract     = {The earliest stages of natural killer (NK) cell development are not well characterized. In this study, we investigated in different fetal hematopoietic tissues how NK cell progenitors and their mature NK cell progeny emerge and expand during fetal development. Here we demonstrate, for the first time, that the counterpart of adult bone marrow Lin(-)CD122(+)NK1.1(-)DX5(-) NK cell progenitor (NKP) emerges in the fetal liver at embryonic day (E) 13.5. Following NKP expansion, immature NK cells emerge at day E14.5 in the liver and E15.5 in the spleen. Thymic NK cells arise at day E15.5, while functionally competent cytotoxic NK cells were present in the liver and spleen at day E16.5 and E17.5, respectively. Fetal NKPs failed to produce B and myeloid cells, but sustained combined NK and T lineage potential at the single cell level. NKPs were also found in the fetal blood, spleen and thymus. These findings demonstrate the emergence and expansion of bipotent NK/T cell progenitor during fetal and adult lymphopoiesis, further supporting that NK/T lineage restriction is taking place prethymically. Uncovering the earliest NK cell developmental stages will provide important clues helping to understand the origin of diverse NK cell subsets, their progenitors and key regulators.},
  author       = {Tang, Yanjuan and Peitzsch, Claudia and NozadCharoudeh, Hojjatollah and Cheng, Min and Chaves Guerrero, Patricia and Jacobsen, Sten Eirik W and Sitnicka Quinn, Ewa},
  issn         = {1528-0020},
  language     = {eng},
  number       = {1},
  pages        = {63--75},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {Emergence of NK cell progenitors and functionally competent NK cell lineage subsets in the early mouse embryo.},
  url          = {http://dx.doi.org/10.1182/blood-2011-02-337980},
  volume       = {120},
  year         = {2012},
}