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Carbon fibre-based microbiosensors for in vivo measurements of acetylcholine and choline

Schuvailo, O N; Dzyadevych, S V; El'skaya, A; Gautier-Sauvigne, S; Csöregi, Elisabeth LU ; Cespuglio, R and Soldatkin, A P (2005) In Biosensors & Bioelectronics 21(1). p.87-94
Abstract
This report describes technical improvements to the manufacture of a carbon fibre electrode for the stable and sensitive detection of H2O2 (detection limit at 0.5 mu M). This electrode was also modified through the co-immobilisation of acetylcholinesterase (AChE) and/or choline oxidase (ChOx) in a bovine serum albumin (BSA) membrane for the development of a sensor for in vivo measurements of acetylcholine and choline. Amperometric measurements were performed using a conventional three-electrode system forming part of a flow-injection set-up at an applied potential of 800-1100 mV relative to an Ag/AgCl reference electrode. The optimised biosensor obtained was reproducible and stable, and exhibited a detection limit of 1 mu M for both... (More)
This report describes technical improvements to the manufacture of a carbon fibre electrode for the stable and sensitive detection of H2O2 (detection limit at 0.5 mu M). This electrode was also modified through the co-immobilisation of acetylcholinesterase (AChE) and/or choline oxidase (ChOx) in a bovine serum albumin (BSA) membrane for the development of a sensor for in vivo measurements of acetylcholine and choline. Amperometric measurements were performed using a conventional three-electrode system forming part of a flow-injection set-up at an applied potential of 800-1100 mV relative to an Ag/AgCl reference electrode. The optimised biosensor obtained was reproducible and stable, and exhibited a detection limit of 1 mu M for both acetylcholine and choline. However, due to the high operating potential used, the biosensor was prone to substantial interference from other electroactive compounds, such as ascorbic acid. Therefore, in a further step, a mediated electron transfer approach was used that incorporated horseradish peroxidase into an osmium-based redox hydrogel layered into the active surface of the electrode. Afterwards, a Nafion layer and a coating containing AChE and/or ChOx co-immobilised in a BSA membrane were successively deposited. This procedure further increased the selectivity of the biosensor, when operated in the same flow-injection system but at an applied potential of -50 mV relative to an Ag/AgCl reference electrode. The sensor exhibited good selectivity and a high sensitivity over a concentration range (0.3-100 mu M) suitable for the measurement of choline and acetylcholine in vivo. (c) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
selectivity, sensitivity, choline, amperometric microbiosensor, carbon fibre electrode, mediated electron, acetylcholine, transfer
in
Biosensors & Bioelectronics
volume
21
issue
1
pages
87 - 94
publisher
Elsevier
external identifiers
  • wos:000232268900010
  • scopus:20444448015
ISSN
1873-4235
DOI
10.1016/j.bios.2004.09.017
language
English
LU publication?
yes
id
86438ea6-a37e-4bc7-bb1a-3737e273509b (old id 222365)
date added to LUP
2007-08-08 08:40:43
date last changed
2017-08-27 05:36:10
@article{86438ea6-a37e-4bc7-bb1a-3737e273509b,
  abstract     = {This report describes technical improvements to the manufacture of a carbon fibre electrode for the stable and sensitive detection of H2O2 (detection limit at 0.5 mu M). This electrode was also modified through the co-immobilisation of acetylcholinesterase (AChE) and/or choline oxidase (ChOx) in a bovine serum albumin (BSA) membrane for the development of a sensor for in vivo measurements of acetylcholine and choline. Amperometric measurements were performed using a conventional three-electrode system forming part of a flow-injection set-up at an applied potential of 800-1100 mV relative to an Ag/AgCl reference electrode. The optimised biosensor obtained was reproducible and stable, and exhibited a detection limit of 1 mu M for both acetylcholine and choline. However, due to the high operating potential used, the biosensor was prone to substantial interference from other electroactive compounds, such as ascorbic acid. Therefore, in a further step, a mediated electron transfer approach was used that incorporated horseradish peroxidase into an osmium-based redox hydrogel layered into the active surface of the electrode. Afterwards, a Nafion layer and a coating containing AChE and/or ChOx co-immobilised in a BSA membrane were successively deposited. This procedure further increased the selectivity of the biosensor, when operated in the same flow-injection system but at an applied potential of -50 mV relative to an Ag/AgCl reference electrode. The sensor exhibited good selectivity and a high sensitivity over a concentration range (0.3-100 mu M) suitable for the measurement of choline and acetylcholine in vivo. (c) 2004 Elsevier B.V. All rights reserved.},
  author       = {Schuvailo, O N and Dzyadevych, S V and El'skaya, A and Gautier-Sauvigne, S and Csöregi, Elisabeth and Cespuglio, R and Soldatkin, A P},
  issn         = {1873-4235},
  keyword      = {selectivity,sensitivity,choline,amperometric microbiosensor,carbon fibre electrode,mediated electron,acetylcholine,transfer},
  language     = {eng},
  number       = {1},
  pages        = {87--94},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Carbon fibre-based microbiosensors for in vivo measurements of acetylcholine and choline},
  url          = {http://dx.doi.org/10.1016/j.bios.2004.09.017},
  volume       = {21},
  year         = {2005},
}