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Growth of arbuscular mycorrhizal mycelium in calcareous dune sand and its interaction with other soil microorganisms as estimated by measurement of specific fatty acids

Olsson, P.A.; Francis, R.; Read, D.J. and Söderström, Bengt LU (1998) In Plant and Soil 201(1). p.9-16
Abstract
Fatty acid analysis was used for determining the extent of the development of the external mycelium of AM fungi (mixed inoculum from a sand dune) growing from roots of Festuca rubra and Plantago lanceolata in calcareous dune sand. The plants were raised in chambers specially designed to permit the growth of AM mycelium in root-free compartments. In two separate experiments, growth of external mycelium in the root-free compartments was detected and the amount of mycelium was estimated using the indicator of AM fungal biomass, phospholipid fatty acid (PLFA) 16:1 omega 5. The results showed that the PLFA 16:1 omega 5 was suitable for estimating the mycelium emerging from the mixed inoculum obtained from the field roots of F: rubra and P... (More)
Fatty acid analysis was used for determining the extent of the development of the external mycelium of AM fungi (mixed inoculum from a sand dune) growing from roots of Festuca rubra and Plantago lanceolata in calcareous dune sand. The plants were raised in chambers specially designed to permit the growth of AM mycelium in root-free compartments. In two separate experiments, growth of external mycelium in the root-free compartments was detected and the amount of mycelium was estimated using the indicator of AM fungal biomass, phospholipid fatty acid (PLFA) 16:1 omega 5. The results showed that the PLFA 16:1 omega 5 was suitable for estimating the mycelium emerging from the mixed inoculum obtained from the field roots of F: rubra and P lanceolata. The PLFA 16:1 omega 5 showed external mycelium to become established in the root-free compartments within a period of 3 weeks and the amount of mycelium to continue to increase at 6 and 9 weeks. Increases in neutral lipid fatty acid (NLFA) 16:1 omega 5 (indicator of storage lipids) over time were inconsistent between the two experiments, but appeared to follow patterns of sporulation in each experiment. In both experiments, the root-free compartment was colonised by saprophytic fungi to a greater extent in the case of non-mycorrhizal than of AM treatment, as indicated by an increase in PLFA 18:2 omega 6,9 (indicator of saprophytic fungi). The absence of an increase in the case of AM treatment indicates that AM fungal mycelium can negatively affect the growth of saprophytic fungi in this soil type. This result was, however, only weakly supported by measurements of ergosterol content. The analysis of bacteria specific PLFAs showed that bacterial biomass was not affected by the AM mycelium. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phospholipids, Glomus fasciculatum, Festuca rubra, neutral lipids, Plantago lanceolata, external mycelium
in
Plant and Soil
volume
201
issue
1
pages
9 - 16
publisher
Springer
external identifiers
  • scopus:0031804726
ISSN
0032-079X
DOI
10.1023/A:1004379404220
language
English
LU publication?
yes
id
f7075b5c-5bdc-4ee3-a42f-d5b05adf9941 (old id 2226222)
date added to LUP
2012-01-16 13:14:50
date last changed
2017-01-01 05:09:58
@article{f7075b5c-5bdc-4ee3-a42f-d5b05adf9941,
  abstract     = {Fatty acid analysis was used for determining the extent of the development of the external mycelium of AM fungi (mixed inoculum from a sand dune) growing from roots of Festuca rubra and Plantago lanceolata in calcareous dune sand. The plants were raised in chambers specially designed to permit the growth of AM mycelium in root-free compartments. In two separate experiments, growth of external mycelium in the root-free compartments was detected and the amount of mycelium was estimated using the indicator of AM fungal biomass, phospholipid fatty acid (PLFA) 16:1 omega 5. The results showed that the PLFA 16:1 omega 5 was suitable for estimating the mycelium emerging from the mixed inoculum obtained from the field roots of F: rubra and P lanceolata. The PLFA 16:1 omega 5 showed external mycelium to become established in the root-free compartments within a period of 3 weeks and the amount of mycelium to continue to increase at 6 and 9 weeks. Increases in neutral lipid fatty acid (NLFA) 16:1 omega 5 (indicator of storage lipids) over time were inconsistent between the two experiments, but appeared to follow patterns of sporulation in each experiment. In both experiments, the root-free compartment was colonised by saprophytic fungi to a greater extent in the case of non-mycorrhizal than of AM treatment, as indicated by an increase in PLFA 18:2 omega 6,9 (indicator of saprophytic fungi). The absence of an increase in the case of AM treatment indicates that AM fungal mycelium can negatively affect the growth of saprophytic fungi in this soil type. This result was, however, only weakly supported by measurements of ergosterol content. The analysis of bacteria specific PLFAs showed that bacterial biomass was not affected by the AM mycelium.},
  author       = {Olsson, P.A. and Francis, R. and Read, D.J. and Söderström, Bengt},
  issn         = {0032-079X},
  keyword      = {phospholipids,Glomus fasciculatum,Festuca rubra,neutral lipids,Plantago
lanceolata,external mycelium},
  language     = {eng},
  number       = {1},
  pages        = {9--16},
  publisher    = {Springer},
  series       = {Plant and Soil},
  title        = {Growth of arbuscular mycorrhizal mycelium in calcareous dune sand and its interaction with other soil microorganisms as estimated by measurement of specific fatty acids},
  url          = {http://dx.doi.org/10.1023/A:1004379404220},
  volume       = {201},
  year         = {1998},
}