Dynamics of cytoskeletal proteins in developing pine ectomycorrhiza
(1996) In Mycorrhiza 6(5). p.423-429- Abstract
- Mycorrhizal short roots of Pinus contorta Dougl. ex Loud colonized by Suillus variegatus (Sow. ex Fr.) O. Kuntze or Paxillus involutus (Batsch) Fr. were collected 1->60 days after fungal contact. The proteins of the inoculated roots were extracted, electrophoretically separated, blotted and immunostained for alpha-tubulin and actin. The development of the mycorrhiza was also followed microscopically. The signal of plant alpha-tubulin was stronger than the signal of fungal alpha-tubulin during the first 5 days in S. variegatus mycorrhiza and was then exceeded by fungal alpha-tubulin. This correlated well with the increase of fungal mycelium in the mycorrhiza. A transient drop in both plant and fungal alpha-tubulin signals was observed 20... (More)
- Mycorrhizal short roots of Pinus contorta Dougl. ex Loud colonized by Suillus variegatus (Sow. ex Fr.) O. Kuntze or Paxillus involutus (Batsch) Fr. were collected 1->60 days after fungal contact. The proteins of the inoculated roots were extracted, electrophoretically separated, blotted and immunostained for alpha-tubulin and actin. The development of the mycorrhiza was also followed microscopically. The signal of plant alpha-tubulin was stronger than the signal of fungal alpha-tubulin during the first 5 days in S. variegatus mycorrhiza and was then exceeded by fungal alpha-tubulin. This correlated well with the increase of fungal mycelium in the mycorrhiza. A transient drop in both plant and fungal alpha-tubulin signals was observed 20 days after fungal contact, suggesting a change in the metabolism of the mycorrhiza. The signals for plant and fungal actins in the mycorrhiza increased steadily during early infection and then remained at a high level as the mycorrhiza matured. Similar trends were observed in P. contorta-P. involutus mycorrhiza. The data from P. contorta-S. variegatus mycorrhizas suggests that alpha-tubulin is a growth-related protein, subject to changes, while the amount of actin reflects the general metabolic activity of the mycorrhiza. In both mycorrhizal systems clear alpha-tubulin and actin signals were detected 60 days after colonization, which indicates that the mycorrhizas were metabolically active in spite of their withered appearance. (Less)
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https://lup.lub.lu.se/record/2226260
- author
- Timonen, Sari ; Söderström, Bengt LU and Raudaskoski, Marjatta
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Actin, Mycorrhiza, Pinus contorta, Suillus variegatus, Tubulin
- in
- Mycorrhiza
- volume
- 6
- issue
- 5
- pages
- 423 - 429
- publisher
- Springer
- external identifiers
-
- scopus:0030469386
- ISSN
- 1432-1890
- DOI
- 10.1007/s005720050142
- language
- English
- LU publication?
- yes
- id
- 7b4803b0-431f-4250-be53-d9442d45bbce (old id 2226260)
- date added to LUP
- 2016-04-01 11:54:22
- date last changed
- 2024-01-08 01:00:45
@article{7b4803b0-431f-4250-be53-d9442d45bbce,
abstract = {{Mycorrhizal short roots of Pinus contorta Dougl. ex Loud colonized by Suillus variegatus (Sow. ex Fr.) O. Kuntze or Paxillus involutus (Batsch) Fr. were collected 1->60 days after fungal contact. The proteins of the inoculated roots were extracted, electrophoretically separated, blotted and immunostained for alpha-tubulin and actin. The development of the mycorrhiza was also followed microscopically. The signal of plant alpha-tubulin was stronger than the signal of fungal alpha-tubulin during the first 5 days in S. variegatus mycorrhiza and was then exceeded by fungal alpha-tubulin. This correlated well with the increase of fungal mycelium in the mycorrhiza. A transient drop in both plant and fungal alpha-tubulin signals was observed 20 days after fungal contact, suggesting a change in the metabolism of the mycorrhiza. The signals for plant and fungal actins in the mycorrhiza increased steadily during early infection and then remained at a high level as the mycorrhiza matured. Similar trends were observed in P. contorta-P. involutus mycorrhiza. The data from P. contorta-S. variegatus mycorrhizas suggests that alpha-tubulin is a growth-related protein, subject to changes, while the amount of actin reflects the general metabolic activity of the mycorrhiza. In both mycorrhizal systems clear alpha-tubulin and actin signals were detected 60 days after colonization, which indicates that the mycorrhizas were metabolically active in spite of their withered appearance.}},
author = {{Timonen, Sari and Söderström, Bengt and Raudaskoski, Marjatta}},
issn = {{1432-1890}},
keywords = {{Actin; Mycorrhiza; Pinus contorta; Suillus variegatus; Tubulin}},
language = {{eng}},
number = {{5}},
pages = {{423--429}},
publisher = {{Springer}},
series = {{Mycorrhiza}},
title = {{Dynamics of cytoskeletal proteins in developing pine ectomycorrhiza}},
url = {{http://dx.doi.org/10.1007/s005720050142}},
doi = {{10.1007/s005720050142}},
volume = {{6}},
year = {{1996}},
}