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Metabolism of [N-15]alanine in the ectomycorrhizal fungus Paxillus involutus

Chalot, Michel; Finlay, Roger D.; Ek, Hans and Söderström, Bengt LU (1995) In Experimental Mycology 19(4). p.297-304
Abstract
Alanine metabolism in the ectomycorrhizal fungus Paxillus involutus was investigated using [N-15]alanine. Short-term exposure of mycelial discs to [N-15]alanine showed that the greatest flow of N-15 was to glutamate and to aspartate. Levels of enrichment were as high as 15-20% for glutamate and 13-18% for aspartate, whereas that of alanine reached 30%. Label was also detected in the amino-N of glutamine and in serine and glycine, although at lower levels. Preincubation of mycelia with aminooxyacetate, an inhibitor of transamination reactions, resulted in complete inhibition of the flow of the label to glutamate, aspartate, and amino-N of glutamine, whereas [N-15]alanine rapidly accumulated. This evidence indicates the direct involvement of... (More)
Alanine metabolism in the ectomycorrhizal fungus Paxillus involutus was investigated using [N-15]alanine. Short-term exposure of mycelial discs to [N-15]alanine showed that the greatest flow of N-15 was to glutamate and to aspartate. Levels of enrichment were as high as 15-20% for glutamate and 13-18% for aspartate, whereas that of alanine reached 30%. Label was also detected in the amino-N of glutamine and in serine and glycine, although at lower levels. Preincubation of mycelia with aminooxyacetate, an inhibitor of transamination reactions, resulted in complete inhibition of the flow of the label to glutamate, aspartate, and amino-N of glutamine, whereas [N-15]alanine rapidly accumulated. This evidence indicates the direct involvement of alanine aminotransferase for translocation of N-15 from alanine to glutamate. Alanine may be a convenient reservoir of both nitrogen and carbon. (C) 1995 Academic Press, Inc. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
alanine metabolism, alanine aminotransferase, aminooxyacetate, ectomycorrhizal fungus, organic nitrogen utilization, Paxillus involutus
in
Experimental Mycology
volume
19
issue
4
pages
297 - 304
publisher
Elsevier
external identifiers
  • scopus:0029589283
ISSN
0140-962X
DOI
10.1006/emyc.1995.1036
language
English
LU publication?
yes
id
59f85ff0-f543-405a-9360-22dc4f3c8061 (old id 2226292)
alternative location
http://www.sciencedirect.com/science/article/pii/S0147597585710365
date added to LUP
2012-01-12 16:54:06
date last changed
2017-07-02 04:24:27
@article{59f85ff0-f543-405a-9360-22dc4f3c8061,
  abstract     = {Alanine metabolism in the ectomycorrhizal fungus Paxillus involutus was investigated using [N-15]alanine. Short-term exposure of mycelial discs to [N-15]alanine showed that the greatest flow of N-15 was to glutamate and to aspartate. Levels of enrichment were as high as 15-20% for glutamate and 13-18% for aspartate, whereas that of alanine reached 30%. Label was also detected in the amino-N of glutamine and in serine and glycine, although at lower levels. Preincubation of mycelia with aminooxyacetate, an inhibitor of transamination reactions, resulted in complete inhibition of the flow of the label to glutamate, aspartate, and amino-N of glutamine, whereas [N-15]alanine rapidly accumulated. This evidence indicates the direct involvement of alanine aminotransferase for translocation of N-15 from alanine to glutamate. Alanine may be a convenient reservoir of both nitrogen and carbon. (C) 1995 Academic Press, Inc.},
  author       = {Chalot, Michel and Finlay, Roger D. and Ek, Hans and Söderström, Bengt},
  issn         = {0140-962X},
  keyword      = {alanine metabolism,alanine aminotransferase,aminooxyacetate,ectomycorrhizal fungus,organic nitrogen utilization,Paxillus involutus},
  language     = {eng},
  number       = {4},
  pages        = {297--304},
  publisher    = {Elsevier},
  series       = {Experimental Mycology},
  title        = {Metabolism of [N-15]alanine in the ectomycorrhizal fungus Paxillus involutus},
  url          = {http://dx.doi.org/10.1006/emyc.1995.1036},
  volume       = {19},
  year         = {1995},
}