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Factors affecting amino-acid-uptake by the ectomycorrhizal fungus Paxillus involutus

Chalot, Michel; Kytoviita, M.M.; Brun, Annick; Finlay, Roger D. and Söderström, Bengt LU (1995) In Mycological Research 99(9). p.1131-1138
Abstract
Factors affecting amino acid uptake by the ectomycorrhizal fungus Paxillus involutus were investigated using tracer kinetic experiments. Amino acid uptake by P. involutus was dependent on the pH of the incubation medium. Uptake of L-glutamate and L-glutamine had a distinct pH optimum around pH 4.0 and declined sharply between pH 4.5 and 6.4. Uptake of alanine and aspartate was also highest at low pH values (3-4) but declined more slowly with increasing pH. Total uptake of labelled amino acids was unaffected by 0.05 or 0.5 mM ammonium while 5 mM ammonium decreased amino acid uptake. Nitrate or glucose did not significantly affect amino acid uptake but glucose greatly increased the proportion of carbon that was respired. Uptake of the... (More)
Factors affecting amino acid uptake by the ectomycorrhizal fungus Paxillus involutus were investigated using tracer kinetic experiments. Amino acid uptake by P. involutus was dependent on the pH of the incubation medium. Uptake of L-glutamate and L-glutamine had a distinct pH optimum around pH 4.0 and declined sharply between pH 4.5 and 6.4. Uptake of alanine and aspartate was also highest at low pH values (3-4) but declined more slowly with increasing pH. Total uptake of labelled amino acids was unaffected by 0.05 or 0.5 mM ammonium while 5 mM ammonium decreased amino acid uptake. Nitrate or glucose did not significantly affect amino acid uptake but glucose greatly increased the proportion of carbon that was respired. Uptake of the different amino acids decreased by a factor of 4 to 10 with increasing age (1-5 wk) and the proportion of absorbed C-14 that was respired increased with age. Changes in the size of the total amino acid pool were inversely related to those in the rate of uptake and the pool increased steadily with age. The implications of the ability of P. involutus to take up amino acids are discussed. (Less)
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Contribution to journal
publication status
published
subject
keywords
Aminoacid, Metabolism, Absorption, Respiration, pH, Glucose, Ammonium, Mycorrhiza, Symbionte, Ectomycorrhiza, Basidiomycetes, Fungi, Thallophyta
in
Mycological Research
volume
99
issue
9
pages
1131 - 1138
publisher
Cambridge University Press
external identifiers
  • scopus:0028877665
ISSN
1469-8102
language
English
LU publication?
yes
id
296f4398-e758-4d99-8139-beff734e24c0 (old id 2226297)
alternative location
http://www.sciencedirect.com/science/article/pii/S0953756209807843
date added to LUP
2012-01-12 14:14:45
date last changed
2017-01-01 05:03:37
@article{296f4398-e758-4d99-8139-beff734e24c0,
  abstract     = {Factors affecting amino acid uptake by the ectomycorrhizal fungus Paxillus involutus were investigated using tracer kinetic experiments. Amino acid uptake by P. involutus was dependent on the pH of the incubation medium. Uptake of L-glutamate and L-glutamine had a distinct pH optimum around pH 4.0 and declined sharply between pH 4.5 and 6.4. Uptake of alanine and aspartate was also highest at low pH values (3-4) but declined more slowly with increasing pH. Total uptake of labelled amino acids was unaffected by 0.05 or 0.5 mM ammonium while 5 mM ammonium decreased amino acid uptake. Nitrate or glucose did not significantly affect amino acid uptake but glucose greatly increased the proportion of carbon that was respired. Uptake of the different amino acids decreased by a factor of 4 to 10 with increasing age (1-5 wk) and the proportion of absorbed C-14 that was respired increased with age. Changes in the size of the total amino acid pool were inversely related to those in the rate of uptake and the pool increased steadily with age. The implications of the ability of P. involutus to take up amino acids are discussed.},
  author       = {Chalot, Michel and Kytoviita, M.M. and Brun, Annick and Finlay, Roger D. and Söderström, Bengt},
  issn         = {1469-8102},
  keyword      = {Aminoacid,Metabolism,Absorption,Respiration,pH,Glucose,Ammonium,Mycorrhiza,Symbionte,Ectomycorrhiza,Basidiomycetes,Fungi,Thallophyta},
  language     = {eng},
  number       = {9},
  pages        = {1131--1138},
  publisher    = {Cambridge University Press},
  series       = {Mycological Research},
  title        = {Factors affecting amino-acid-uptake by the ectomycorrhizal fungus Paxillus involutus},
  volume       = {99},
  year         = {1995},
}