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The use of phospholipid and neutral lipid fatty-acids to estimate biomass of arbuscular mycorrhizal fungi in soil

Olsson, P.A.,; Bååth, Erland LU ; Jakobsen, I., and Söderström, Bengt LU (1995) In Mycological Research 99(5). p.623-629
Abstract
Cucumber seedlings associated with the arbuscular mycorrhizal (AM) fungi Glomus WUM10 or G. caledanium (BEG 15) were grown in PVC tubes with a lateral root-free compartment and an identical compartment containing both hyphae and roots. The amounts of specific fatty acids, in the neutral lipid and phospholipid fractions, were measured in both compartments and compared with controls without mycorrhiza. The phospholipid fatty acids (PLFAs) 16:1 omega 5, 18:1 omega 7c, 20:4 and 20:5 were present in higher amounts in soil with mycorrhizal hyphae than in soil without mycorrhizal hyphae. The largest relative difference was found in 20:5, but a good correlation existed between 16:1 omega 5 and 20:5 in soil with hyphae. Amounts of these fatty acids... (More)
Cucumber seedlings associated with the arbuscular mycorrhizal (AM) fungi Glomus WUM10 or G. caledanium (BEG 15) were grown in PVC tubes with a lateral root-free compartment and an identical compartment containing both hyphae and roots. The amounts of specific fatty acids, in the neutral lipid and phospholipid fractions, were measured in both compartments and compared with controls without mycorrhiza. The phospholipid fatty acids (PLFAs) 16:1 omega 5, 18:1 omega 7c, 20:4 and 20:5 were present in higher amounts in soil with mycorrhizal hyphae than in soil without mycorrhizal hyphae. The largest relative difference was found in 20:5, but a good correlation existed between 16:1 omega 5 and 20:5 in soil with hyphae. Amounts of these fatty acids were correlated both with length of mycorrhizal hyphae and with amounts of ATP in soil. Conversion factors to calculate hyphal length and AM fungal biomass carbon using the phospholipid fatty acids could thus be estimated; 38 nmol PLFA 16:1 omega 5 mg(-1) AM fungal biomass C (Glomus WUM10) and 22 nmol PLFA 20:5 mg(-1) biomass C. The fatty acid 16:1 omega 5 from the neutral lipid fraction, containing triglycerides, dominated in soils with mycorrhizal hyphae. The amount of 16:1 omega 5 in the neutral lipid fraction decreased during storage of soils, indicating a decrease in storage lipids, while the proportion of 16:1 omega 5 in the phospholipid fraction was almost unaffected. (Less)
Please use this url to cite or link to this publication:
author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Biomass, Endomycorrhiza, Specific identification, Cucumis sativus, Neutral lipid, Fatty acids, Phospholipid, ATP, Investigation method, Cucurbitaceae, Dicotyledones, Angiospermae, Spermatophyta, Symbionte, Phycomycetes, Fungi, Thallophyta
in
Mycological Research
volume
99
issue
5
pages
623 - 629
publisher
Cambridge University Press
external identifiers
  • scopus:0029107737
ISSN
1469-8102
language
English
LU publication?
yes
id
c12ecfb2-e0a8-4858-9708-8cd7a161badf (old id 2226303)
alternative location
http://www.sciencedirect.com/science/article/pii/S0953756209807235
date added to LUP
2012-01-11 16:47:13
date last changed
2017-10-29 03:24:26
@article{c12ecfb2-e0a8-4858-9708-8cd7a161badf,
  abstract     = {Cucumber seedlings associated with the arbuscular mycorrhizal (AM) fungi Glomus WUM10 or G. caledanium (BEG 15) were grown in PVC tubes with a lateral root-free compartment and an identical compartment containing both hyphae and roots. The amounts of specific fatty acids, in the neutral lipid and phospholipid fractions, were measured in both compartments and compared with controls without mycorrhiza. The phospholipid fatty acids (PLFAs) 16:1 omega 5, 18:1 omega 7c, 20:4 and 20:5 were present in higher amounts in soil with mycorrhizal hyphae than in soil without mycorrhizal hyphae. The largest relative difference was found in 20:5, but a good correlation existed between 16:1 omega 5 and 20:5 in soil with hyphae. Amounts of these fatty acids were correlated both with length of mycorrhizal hyphae and with amounts of ATP in soil. Conversion factors to calculate hyphal length and AM fungal biomass carbon using the phospholipid fatty acids could thus be estimated; 38 nmol PLFA 16:1 omega 5 mg(-1) AM fungal biomass C (Glomus WUM10) and 22 nmol PLFA 20:5 mg(-1) biomass C. The fatty acid 16:1 omega 5 from the neutral lipid fraction, containing triglycerides, dominated in soils with mycorrhizal hyphae. The amount of 16:1 omega 5 in the neutral lipid fraction decreased during storage of soils, indicating a decrease in storage lipids, while the proportion of 16:1 omega 5 in the phospholipid fraction was almost unaffected.},
  author       = {Olsson, P.A., and Bååth, Erland and Jakobsen, I., and Söderström, Bengt},
  issn         = {1469-8102},
  keyword      = {Biomass,Endomycorrhiza,Specific identification,Cucumis sativus,Neutral lipid,Fatty acids,Phospholipid,ATP,Investigation method,Cucurbitaceae,Dicotyledones,Angiospermae,Spermatophyta,Symbionte,Phycomycetes,Fungi,Thallophyta},
  language     = {eng},
  number       = {5},
  pages        = {623--629},
  publisher    = {Cambridge University Press},
  series       = {Mycological Research},
  title        = {The use of phospholipid and neutral lipid fatty-acids to estimate biomass of arbuscular mycorrhizal fungi in soil},
  volume       = {99},
  year         = {1995},
}