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Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus

Chalot, Michel; Brun, Annick; Finlay, Roger D. and Söderström, Bengt LU (1994) In FEMS Microbiology Letters 121(1). p.87-91
Abstract
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino acid respiration, Aminooxyacetate, [14C]alanine, Ectomycorrhizal fungus, Paxillus involutus, TCA cycle
in
FEMS Microbiology Letters
volume
121
issue
1
pages
87 - 91
publisher
Elsevier
ISSN
1574-6968
DOI
10.1111/j.1574-6968.1994.tb07080.x
language
English
LU publication?
yes
id
413b9026-d67e-4c8a-9cc8-ed72ef940e5b (old id 2226321)
date added to LUP
2012-01-11 16:01:17
date last changed
2016-04-15 18:47:51
@article{413b9026-d67e-4c8a-9cc8-ed72ef940e5b,
  abstract     = {The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.},
  author       = {Chalot, Michel and Brun, Annick and Finlay, Roger D. and Söderström, Bengt},
  issn         = {1574-6968},
  keyword      = {Amino acid respiration,Aminooxyacetate,[14C]alanine,Ectomycorrhizal fungus,Paxillus involutus,TCA cycle},
  language     = {eng},
  number       = {1},
  pages        = {87--91},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.1994.tb07080.x},
  volume       = {121},
  year         = {1994},
}