Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus
(1994) In FEMS Microbiology Letters 121(1). p.87-91- Abstract
- The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2226321
- author
- Chalot, Michel ; Brun, Annick ; Finlay, Roger D. and Söderström, Bengt LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amino acid respiration, Aminooxyacetate, [14C]alanine, Ectomycorrhizal fungus, Paxillus involutus, TCA cycle
- in
- FEMS Microbiology Letters
- volume
- 121
- issue
- 1
- pages
- 87 - 91
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0028176327
- ISSN
- 1574-6968
- DOI
- 10.1111/j.1574-6968.1994.tb07080.x
- language
- English
- LU publication?
- yes
- id
- 413b9026-d67e-4c8a-9cc8-ed72ef940e5b (old id 2226321)
- date added to LUP
- 2016-04-01 11:40:18
- date last changed
- 2024-01-07 16:02:49
@article{413b9026-d67e-4c8a-9cc8-ed72ef940e5b, abstract = {{The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.}}, author = {{Chalot, Michel and Brun, Annick and Finlay, Roger D. and Söderström, Bengt}}, issn = {{1574-6968}}, keywords = {{Amino acid respiration; Aminooxyacetate; [14C]alanine; Ectomycorrhizal fungus; Paxillus involutus; TCA cycle}}, language = {{eng}}, number = {{1}}, pages = {{87--91}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus}}, url = {{http://dx.doi.org/10.1111/j.1574-6968.1994.tb07080.x}}, doi = {{10.1111/j.1574-6968.1994.tb07080.x}}, volume = {{121}}, year = {{1994}}, }