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Determination of N-15-labeled ammonium and total nitrogen in plant and fungal systems using mass-spectrometry

Ek, H.; Finlay, R.D.; Söderström, Bengt LU and Odham, G. (1990) In Journal of Microbiological Methods 11(3-4). p.169-176
Abstract
A selected ion-monitoring method to measure 15N-labelled ammonia in biological samples was improved to simplify sample handling, to obviate interference from ammonia due to the decomposition of glutamine

and to allow the determination of total N. Ammonia is derivatized with pentafluorobenzoylchloride to yield pentafluorobenzamide which is analysed by gas chromatography/mass spectrometry after clean-up using

disposable silicic acid columns. The sensitivity achieved when operating in the negative ion-chemical ionization mode was somewhat higher than when using electron-impact ionization. Use of methyl amine as an internal standard improved the accuracy and precision of the measurements. The method was applied to samples... (More)
A selected ion-monitoring method to measure 15N-labelled ammonia in biological samples was improved to simplify sample handling, to obviate interference from ammonia due to the decomposition of glutamine

and to allow the determination of total N. Ammonia is derivatized with pentafluorobenzoylchloride to yield pentafluorobenzamide which is analysed by gas chromatography/mass spectrometry after clean-up using

disposable silicic acid columns. The sensitivity achieved when operating in the negative ion-chemical ionization mode was somewhat higher than when using electron-impact ionization. Use of methyl amine as an internal standard improved the accuracy and precision of the measurements. The method was applied to samples taken from an intact ectomycorrhizal system fed with ISN-labelled ammonium and used to determine patterns of N assimilation into ammonium, free amino acids and macromolecular compounds. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ammonia, Ectomycorrhiza, Mass spectrometry, 15N, N assimilation
in
Journal of Microbiological Methods
volume
11
issue
3-4
pages
169 - 176
publisher
Elsevier
external identifiers
  • scopus:0025324302
ISSN
1872-8359
language
English
LU publication?
yes
id
4964c2e8-f532-48a7-9552-3a718f29df16 (old id 2226370)
alternative location
http://www.sciencedirect.com/science/article/pii/0167701290900539
date added to LUP
2012-01-11 13:38:58
date last changed
2017-01-01 04:34:22
@article{4964c2e8-f532-48a7-9552-3a718f29df16,
  abstract     = {A selected ion-monitoring method to measure 15N-labelled ammonia in biological samples was improved to simplify sample handling, to obviate interference from ammonia due to the decomposition of glutamine<br/><br>
and to allow the determination of total N. Ammonia is derivatized with pentafluorobenzoylchloride to yield pentafluorobenzamide which is analysed by gas chromatography/mass spectrometry after clean-up using<br/><br>
disposable silicic acid columns. The sensitivity achieved when operating in the negative ion-chemical ionization mode was somewhat higher than when using electron-impact ionization. Use of methyl amine as an internal standard improved the accuracy and precision of the measurements. The method was applied to samples taken from an intact ectomycorrhizal system fed with ISN-labelled ammonium and used to determine patterns of N assimilation into ammonium, free amino acids and macromolecular compounds.},
  author       = {Ek, H. and Finlay, R.D. and Söderström, Bengt and Odham, G.},
  issn         = {1872-8359},
  keyword      = {Ammonia,Ectomycorrhiza,Mass spectrometry,15N,N assimilation},
  language     = {eng},
  number       = {3-4},
  pages        = {169--176},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {Determination of N-15-labeled ammonium and total nitrogen in plant and fungal systems using mass-spectrometry},
  volume       = {11},
  year         = {1990},
}