Advanced

Mycelial uptake, translocation and assimilation of N-15-labeled nitrogen by ectomycorrhizal Pinus sylvestris plants

Finlay, R.D.; Ek, H.; Odham, G. and Söderström, Bengt LU (1990) In Agriculture, Ecosystems & Environment 28(1-4). p.133-137
Abstract
Mycolial uptake, translocation and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris plants infected with the ectomycorrhizal fungus. Paxillus involutus. The distribution of labelled compounds within the systems was examined using gas chromatography / mass spectrometry. Labelled nitrogen was incorporated into a range of free amino acids in the fungal mycelium, the principal sinks being alanine and glutamatelglutamine. Levels of 15 N enrichment declined throughout the transport pathway from 30-45% in the mycelium a~d mycorrhlzal root lips to 3-11% in the plant shoots. A significant proportion of the total assimilated nitrogen (27%) was incorporated into

proteinaceous material and the proportion of label present... (More)
Mycolial uptake, translocation and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris plants infected with the ectomycorrhizal fungus. Paxillus involutus. The distribution of labelled compounds within the systems was examined using gas chromatography / mass spectrometry. Labelled nitrogen was incorporated into a range of free amino acids in the fungal mycelium, the principal sinks being alanine and glutamatelglutamine. Levels of 15 N enrichment declined throughout the transport pathway from 30-45% in the mycelium a~d mycorrhlzal root lips to 3-11% in the plant shoots. A significant proportion of the total assimilated nitrogen (27%) was incorporated into

proteinaceous material and the proportion of label present in this fraction increased from 27% in the mycelium to 32% and 58% in the plant roots and needles respectively. The protein-incorporated amino acids showed a wider spectrum of labelling with significant amounts of 15N incorporated into valine, isoleucine, phenylalanine and lysine. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Agriculture, Ecosystems & Environment
volume
28
issue
1-4
pages
133 - 137
publisher
Elsevier
ISSN
1873-2305
language
English
LU publication?
yes
id
df20d585-6501-4510-97d9-281672b1c6cf (old id 2226382)
alternative location
http://www.sciencedirect.com/science/article/pii/016788099090028C
date added to LUP
2012-01-11 13:19:41
date last changed
2016-04-15 19:20:52
@article{df20d585-6501-4510-97d9-281672b1c6cf,
  abstract     = {Mycolial uptake, translocation and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris plants infected with the ectomycorrhizal fungus. Paxillus involutus. The distribution of labelled compounds within the systems was examined using gas chromatography / mass spectrometry. Labelled nitrogen was incorporated into a range of free amino acids in the fungal mycelium, the principal sinks being alanine and glutamatelglutamine. Levels of 15 N enrichment declined throughout the transport pathway from 30-45% in the mycelium a~d mycorrhlzal root lips to 3-11% in the plant shoots. A significant proportion of the total assimilated nitrogen (27%) was incorporated into<br/><br>
proteinaceous material and the proportion of label present in this fraction increased from 27% in the mycelium to 32% and 58% in the plant roots and needles respectively. The protein-incorporated amino acids showed a wider spectrum of labelling with significant amounts of 15N incorporated into valine, isoleucine, phenylalanine and lysine.},
  author       = {Finlay, R.D. and Ek, H. and Odham, G. and Söderström, Bengt},
  issn         = {1873-2305},
  language     = {eng},
  number       = {1-4},
  pages        = {133--137},
  publisher    = {Elsevier},
  series       = {Agriculture, Ecosystems & Environment},
  title        = {Mycelial uptake, translocation and assimilation of N-15-labeled nitrogen by ectomycorrhizal Pinus sylvestris plants},
  volume       = {28},
  year         = {1990},
}