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Mycelial uptake, translocation and assimilation of N-15-labeled nitrogen by ectomycorrhizal Pinus sylvestris plants

Finlay, R.D. ; Ek, H. ; Odham, G. and Söderström, Bengt LU (1990) In Agriculture, Ecosystems & Environment 28(1-4). p.133-137
Abstract
Mycolial uptake, translocation and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris plants infected with the ectomycorrhizal fungus. Paxillus involutus. The distribution of labelled compounds within the systems was examined using gas chromatography / mass spectrometry. Labelled nitrogen was incorporated into a range of free amino acids in the fungal mycelium, the principal sinks being alanine and glutamatelglutamine. Levels of 15 N enrichment declined throughout the transport pathway from 30-45% in the mycelium a~d mycorrhlzal root lips to 3-11% in the plant shoots. A significant proportion of the total assimilated nitrogen (27%) was incorporated into

proteinaceous material and the proportion of label present... (More)
Mycolial uptake, translocation and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris plants infected with the ectomycorrhizal fungus. Paxillus involutus. The distribution of labelled compounds within the systems was examined using gas chromatography / mass spectrometry. Labelled nitrogen was incorporated into a range of free amino acids in the fungal mycelium, the principal sinks being alanine and glutamatelglutamine. Levels of 15 N enrichment declined throughout the transport pathway from 30-45% in the mycelium a~d mycorrhlzal root lips to 3-11% in the plant shoots. A significant proportion of the total assimilated nitrogen (27%) was incorporated into

proteinaceous material and the proportion of label present in this fraction increased from 27% in the mycelium to 32% and 58% in the plant roots and needles respectively. The protein-incorporated amino acids showed a wider spectrum of labelling with significant amounts of 15N incorporated into valine, isoleucine, phenylalanine and lysine. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Agriculture, Ecosystems & Environment
volume
28
issue
1-4
pages
133 - 137
publisher
Elsevier
ISSN
1873-2305
language
English
LU publication?
yes
id
df20d585-6501-4510-97d9-281672b1c6cf (old id 2226382)
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http://www.sciencedirect.com/science/article/pii/016788099090028C
date added to LUP
2016-04-01 11:55:33
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2020-09-23 15:14:16
@article{df20d585-6501-4510-97d9-281672b1c6cf,
  abstract     = {{Mycolial uptake, translocation and assimilation of 15N-labelled ammonium was followed in Pinus sylvestris plants infected with the ectomycorrhizal fungus. Paxillus involutus. The distribution of labelled compounds within the systems was examined using gas chromatography / mass spectrometry. Labelled nitrogen was incorporated into a range of free amino acids in the fungal mycelium, the principal sinks being alanine and glutamatelglutamine. Levels of 15 N enrichment declined throughout the transport pathway from 30-45% in the mycelium a~d mycorrhlzal root lips to 3-11% in the plant shoots. A significant proportion of the total assimilated nitrogen (27%) was incorporated into<br/><br>
proteinaceous material and the proportion of label present in this fraction increased from 27% in the mycelium to 32% and 58% in the plant roots and needles respectively. The protein-incorporated amino acids showed a wider spectrum of labelling with significant amounts of 15N incorporated into valine, isoleucine, phenylalanine and lysine.}},
  author       = {{Finlay, R.D. and Ek, H. and Odham, G. and Söderström, Bengt}},
  issn         = {{1873-2305}},
  language     = {{eng}},
  number       = {{1-4}},
  pages        = {{133--137}},
  publisher    = {{Elsevier}},
  series       = {{Agriculture, Ecosystems & Environment}},
  title        = {{Mycelial uptake, translocation and assimilation of N-15-labeled nitrogen by ectomycorrhizal Pinus sylvestris plants}},
  url          = {{http://www.sciencedirect.com/science/article/pii/016788099090028C}},
  volume       = {{28}},
  year         = {{1990}},
}