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The structure and function of the vegetative mycelium of ectomycorrhizal plants .3. Ultrastructural and autoradiographic analysis of inter-plant carbon distribution through intact mycelial systems

Duddridge, J.A.; Finlay, R.D.; Read, D.J. and Söderström, Bengt LU (1988) In New Phytologist 108(2). p.183-188
Abstract
Plants of Pinus sylvestris L. were grown in mycorrhizal association with Suillus bovinus (Fr.) O. Kuntze in

observation chambers until an interconnecting mycelial network had developed between the seedlings. The shoot

of an individual seedling was then sealed in a perspex cuvette and exposed to 14CO2. After incubation for 48 h, the

'donor' shoot was removed and components of the mycelial network and mycorrhizal roots of connected and

unconnected 'receiver' seedlings were harvested, fixed, embedded and sectioned prior to being autoradiographed

and examined by transmission electron microscopy. The patterns of distribution of 14C-labelled material within

the mycelial strands, the sheath... (More)
Plants of Pinus sylvestris L. were grown in mycorrhizal association with Suillus bovinus (Fr.) O. Kuntze in

observation chambers until an interconnecting mycelial network had developed between the seedlings. The shoot

of an individual seedling was then sealed in a perspex cuvette and exposed to 14CO2. After incubation for 48 h, the

'donor' shoot was removed and components of the mycelial network and mycorrhizal roots of connected and

unconnected 'receiver' seedlings were harvested, fixed, embedded and sectioned prior to being autoradiographed

and examined by transmission electron microscopy. The patterns of distribution of 14C-labelled material within

the mycelial strands, the sheath and root tissues was examined. The results are discussed in relation to carbon

metabolism of the fungus and of the interconnected host plant. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ectomycorrhiza, carbon pathway, micro-autoradiography, ultrastructure, Pinus sylvestris
in
New Phytologist
volume
108
issue
2
pages
183 - 188
publisher
Wiley-Blackwell
ISSN
1469-8137
language
English
LU publication?
yes
id
7db6df5a-d9ef-4b7f-97c1-3f3578597fa1 (old id 2226417)
alternative location
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.1988.tb03695.x/pdf
date added to LUP
2012-01-10 16:13:18
date last changed
2016-04-15 18:46:03
@article{7db6df5a-d9ef-4b7f-97c1-3f3578597fa1,
  abstract     = {Plants of Pinus sylvestris L. were grown in mycorrhizal association with Suillus bovinus (Fr.) O. Kuntze in<br/><br>
observation chambers until an interconnecting mycelial network had developed between the seedlings. The shoot<br/><br>
of an individual seedling was then sealed in a perspex cuvette and exposed to 14CO2. After incubation for 48 h, the<br/><br>
'donor' shoot was removed and components of the mycelial network and mycorrhizal roots of connected and<br/><br>
unconnected 'receiver' seedlings were harvested, fixed, embedded and sectioned prior to being autoradiographed<br/><br>
and examined by transmission electron microscopy. The patterns of distribution of 14C-labelled material within<br/><br>
the mycelial strands, the sheath and root tissues was examined. The results are discussed in relation to carbon<br/><br>
metabolism of the fungus and of the interconnected host plant.},
  author       = {Duddridge, J.A. and Finlay, R.D. and Read, D.J. and Söderström, Bengt},
  issn         = {1469-8137},
  keyword      = {Ectomycorrhiza,carbon pathway,micro-autoradiography,ultrastructure,Pinus sylvestris},
  language     = {eng},
  number       = {2},
  pages        = {183--188},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {The structure and function of the vegetative mycelium of ectomycorrhizal plants .3. Ultrastructural and autoradiographic analysis of inter-plant carbon distribution through intact mycelial systems},
  volume       = {108},
  year         = {1988},
}