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Growth of Verticillium dahliae Kleb. hyphae and of bacteria along the roots of rape (Brassica napus L.) seedlings

Olsson, Stefan; Bååth, Erland LU and Söderström, Bengt LU (1987) In Canadian Journal of Microbiology 33(10). p.916-919
Abstract
Bacterial density and the hyphal density of Verticillium dahliae were determined along the roots of sand-grown rape seedlings. Verticillium dahliae microsclerotia were trapped in nylon nets which were placed in front of the growing root tip. The growing hyphae on the nets were stained with fluorescein diacetate and rhodamine B isothiocyanate. Bacteria on the petri dish lids adjacent to the nets were stained with acridine orange. Both fungal and bacterial densities increased exponentially over a distance of 15–20 mm from the root tip. Further back along the root, the densities of microorganisms stabilized, but in some cases the fluorescein diacetate active hyphal density increased. The hyphal densities stopped increasing before bacterial... (More)
Bacterial density and the hyphal density of Verticillium dahliae were determined along the roots of sand-grown rape seedlings. Verticillium dahliae microsclerotia were trapped in nylon nets which were placed in front of the growing root tip. The growing hyphae on the nets were stained with fluorescein diacetate and rhodamine B isothiocyanate. Bacteria on the petri dish lids adjacent to the nets were stained with acridine orange. Both fungal and bacterial densities increased exponentially over a distance of 15–20 mm from the root tip. Further back along the root, the densities of microorganisms stabilized, but in some cases the fluorescein diacetate active hyphal density increased. The hyphal densities stopped increasing before bacterial densities, suggesting an increasing bacterial competition for root exudates as their population grew. The hyphal activity, measured as the proportion of flurescein diacetate to rhodamine B isothioicyanate stained hyphae, increased during the exponential growth phase of the bacteria, then decreased, and finally increased again as the lateral roots appeared. (Less)
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published
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in
Canadian Journal of Microbiology
volume
33
issue
10
pages
916 - 919
publisher
National Research Council Canada
ISSN
0008-4166
language
English
LU publication?
yes
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afda42d6-305e-4232-bf09-c37e192f21dd (old id 2226431)
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2012-01-10 14:22:26
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2016-04-15 18:47:45
@article{afda42d6-305e-4232-bf09-c37e192f21dd,
  abstract     = {Bacterial density and the hyphal density of Verticillium dahliae were determined along the roots of sand-grown rape seedlings. Verticillium dahliae microsclerotia were trapped in nylon nets which were placed in front of the growing root tip. The growing hyphae on the nets were stained with fluorescein diacetate and rhodamine B isothiocyanate. Bacteria on the petri dish lids adjacent to the nets were stained with acridine orange. Both fungal and bacterial densities increased exponentially over a distance of 15–20 mm from the root tip. Further back along the root, the densities of microorganisms stabilized, but in some cases the fluorescein diacetate active hyphal density increased. The hyphal densities stopped increasing before bacterial densities, suggesting an increasing bacterial competition for root exudates as their population grew. The hyphal activity, measured as the proportion of flurescein diacetate to rhodamine B isothioicyanate stained hyphae, increased during the exponential growth phase of the bacteria, then decreased, and finally increased again as the lateral roots appeared.},
  author       = {Olsson, Stefan and Bååth, Erland and Söderström, Bengt},
  issn         = {0008-4166},
  language     = {eng},
  number       = {10},
  pages        = {916--919},
  publisher    = {National Research Council Canada},
  series       = {Canadian Journal of Microbiology},
  title        = {Growth of Verticillium dahliae Kleb. hyphae and of bacteria along the roots of rape (Brassica napus L.) seedlings},
  volume       = {33},
  year         = {1987},
}