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Vital staining of fungi in pure cultures and in soil with fluorescein diacetate

Söderström, Bengt LU (1977) In Soil Biology & Biochemistry 9(1). p.59-63
Abstract
A method for vital staining of fungal mycelium with fluorescein diacetate (FDA) is described. In experiments with pure cultures, good correlation was obtained between relative staining efficiency, growth rate, and respiration. FDA thus appears to be a true vital stain, in that it stains only metabolically active mycelia.



In fresh soil suspensions stained with FDA, brightly fluorescent hyphae and portions of hyphae were observed. The applicability of the method for measurement of active mycelium in the soil is discussed.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soil Biology & Biochemistry
volume
9
issue
1
pages
59 - 63
publisher
Elsevier
external identifiers
  • scopus:49349130411
ISSN
0038-0717
DOI
10.1016/0038-0717(77)90061-X
language
English
LU publication?
yes
id
bca5402b-bc9f-438a-b046-4ac91be5bf09 (old id 2226598)
date added to LUP
2016-04-01 12:06:35
date last changed
2024-04-09 01:09:47
@article{bca5402b-bc9f-438a-b046-4ac91be5bf09,
  abstract     = {{A method for vital staining of fungal mycelium with fluorescein diacetate (FDA) is described. In experiments with pure cultures, good correlation was obtained between relative staining efficiency, growth rate, and respiration. FDA thus appears to be a true vital stain, in that it stains only metabolically active mycelia.<br/><br>
<br/><br>
In fresh soil suspensions stained with FDA, brightly fluorescent hyphae and portions of hyphae were observed. The applicability of the method for measurement of active mycelium in the soil is discussed.}},
  author       = {{Söderström, Bengt}},
  issn         = {{0038-0717}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{59--63}},
  publisher    = {{Elsevier}},
  series       = {{Soil Biology & Biochemistry}},
  title        = {{Vital staining of fungi in pure cultures and in soil with fluorescein diacetate}},
  url          = {{http://dx.doi.org/10.1016/0038-0717(77)90061-X}},
  doi          = {{10.1016/0038-0717(77)90061-X}},
  volume       = {{9}},
  year         = {{1977}},
}