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Labeling plasmin with technetium-99m for scintigraphic localization of thrombi

Persson, Bertil R.R. LU and Darte, Lennart LU (1977) In The International Journal Of Applied Radiation And Isotopes 28(1-2). p.97-104
Abstract

A detailed study has been made of the method for labeling plasmin (NOVO Indstri A/S Denmark) with 99mTc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing 99mTc-plasmic involved the reduction of 2.5 ml 99mTc-pertechnetate solution with 0.5 ml of 4 mM SnCl2, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl2, NaCl, plasmin and lysine were studied. The enzymatic activity... (More)

A detailed study has been made of the method for labeling plasmin (NOVO Indstri A/S Denmark) with 99mTc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing 99mTc-plasmic involved the reduction of 2.5 ml 99mTc-pertechnetate solution with 0.5 ml of 4 mM SnCl2, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl2, NaCl, plasmin and lysine were studied. The enzymatic activity of plasmin was reduced by less than 15% by the labeling process. Preliminary experiments in rabbits with artificially induced thrombi indicate accumulation of 99mTc activity in the same areas as 125I-fibrinogen after the administration of 99mTc-plasmin.

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Contribution to journal
publication status
published
in
The International Journal Of Applied Radiation And Isotopes
volume
28
issue
1-2
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:140151
  • scopus:0017367663
ISSN
0020-708X
DOI
10.1016/0020-708X(77)90163-6
language
English
LU publication?
yes
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222ff1e2-4211-40af-9ca7-ad69d496ac9c
date added to LUP
2020-08-30 21:02:53
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2020-09-03 14:02:08
@article{222ff1e2-4211-40af-9ca7-ad69d496ac9c,
  abstract     = {<p>A detailed study has been made of the method for labeling plasmin (NOVO Indstri A/S Denmark) with <sup>99m</sup>Tc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing <sup>99m</sup>Tc-plasmic involved the reduction of 2.5 ml <sup>99m</sup>Tc-pertechnetate solution with 0.5 ml of 4 mM SnCl<sub>2</sub>, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl<sub>2</sub>, NaCl, plasmin and lysine were studied. The enzymatic activity of plasmin was reduced by less than 15% by the labeling process. Preliminary experiments in rabbits with artificially induced thrombi indicate accumulation of <sup>99m</sup>Tc activity in the same areas as <sup>125</sup>I-fibrinogen after the administration of <sup>99m</sup>Tc-plasmin.</p>},
  author       = {Persson, Bertil R.R. and Darte, Lennart},
  issn         = {0020-708X},
  language     = {eng},
  month        = {01},
  number       = {1-2},
  pages        = {97--104},
  publisher    = {Elsevier},
  series       = {The International Journal Of Applied Radiation And Isotopes},
  title        = {Labeling plasmin with technetium-99m for scintigraphic localization of thrombi},
  url          = {http://dx.doi.org/10.1016/0020-708X(77)90163-6},
  doi          = {10.1016/0020-708X(77)90163-6},
  volume       = {28},
  year         = {1977},
}