Cytokeratin 5/14-positive breast cancer: true basal phenotype confined to BRCA1 tumors
(2005) In Modern Pathology 18(10). p.1321-1328- Abstract
- Breast ducts contain two types of epithelial cells, inner luminal cells and outer basal/ myoepithelial cells. These cells can be distinguished by their immunophenotype. Cytokeratins ( CKs) 8 and 18 are expressed in the luminal layer, whereas CK5/ 14 and the transcription factor p63 characterize the basal epithelial layer. We studied a population- based cohort of 288 sporadic ductal invasive cancers and found 9% positive for CK5/ 14 and 4% positive for p63. Using a highly sensitive polymer- based immunohistochemical staining, all sporadic tumors were positive for the luminal CK8/ 18, including those positive for CK5/ 14. Pairs of primary tumors and metastases ( n = 38) were always concordant for CK5/ 14 expression. The majority of the CK5/... (More)
- Breast ducts contain two types of epithelial cells, inner luminal cells and outer basal/ myoepithelial cells. These cells can be distinguished by their immunophenotype. Cytokeratins ( CKs) 8 and 18 are expressed in the luminal layer, whereas CK5/ 14 and the transcription factor p63 characterize the basal epithelial layer. We studied a population- based cohort of 288 sporadic ductal invasive cancers and found 9% positive for CK5/ 14 and 4% positive for p63. Using a highly sensitive polymer- based immunohistochemical staining, all sporadic tumors were positive for the luminal CK8/ 18, including those positive for CK5/ 14. Pairs of primary tumors and metastases ( n = 38) were always concordant for CK5/ 14 expression. The majority of the CK5/ 14- positive cases were of histologic grade III ( P = 0.0007) and steroid hormone receptor negative ( P < 0.0001). CK5/ 14 expression was inversely associated with HER- 2 oncogene amplification, but only in the subgroup of estrogen receptor-negative tumors ( P = 0.007). In a separate set of 42 hereditary breast cancers, the majority ( 78%) of the BRCA1-associated tumors, but only one of 15 BRCA2- associated tumors was positive for CK5/ 14. In contrast to sporadic CK5/ 14- positive tumors, BRCA1- associated tumors displayed less intense CK8/ 18 staining, including some truly CK5/ 14- positive CK8/ 18- negative cases. These results suggest that CK5/ 14- positive sporadic breast cancers arise from glandularly committed progenitor cells rather than true CK8/ 18- negative basal cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/223066
- author
- Laakso, M ; Loman, Niklas LU ; Borg, Åke LU and Isola, J
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immunohistochemistry, HER-2, cytokeratin, BRCA2, basal phenotype, BRCA1, oncogene, progenitor cell
- in
- Modern Pathology
- volume
- 18
- issue
- 10
- pages
- 1321 - 1328
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000232145600006
- pmid:15990899
- scopus:25144516817
- pmid:15990899
- ISSN
- 1530-0285
- DOI
- 10.1038/modpathol.3800456
- language
- English
- LU publication?
- yes
- id
- 60793369-481c-446e-ba9e-489cf6fd5746 (old id 223066)
- date added to LUP
- 2016-04-01 16:50:51
- date last changed
- 2022-03-22 21:35:31
@article{60793369-481c-446e-ba9e-489cf6fd5746, abstract = {{Breast ducts contain two types of epithelial cells, inner luminal cells and outer basal/ myoepithelial cells. These cells can be distinguished by their immunophenotype. Cytokeratins ( CKs) 8 and 18 are expressed in the luminal layer, whereas CK5/ 14 and the transcription factor p63 characterize the basal epithelial layer. We studied a population- based cohort of 288 sporadic ductal invasive cancers and found 9% positive for CK5/ 14 and 4% positive for p63. Using a highly sensitive polymer- based immunohistochemical staining, all sporadic tumors were positive for the luminal CK8/ 18, including those positive for CK5/ 14. Pairs of primary tumors and metastases ( n = 38) were always concordant for CK5/ 14 expression. The majority of the CK5/ 14- positive cases were of histologic grade III ( P = 0.0007) and steroid hormone receptor negative ( P < 0.0001). CK5/ 14 expression was inversely associated with HER- 2 oncogene amplification, but only in the subgroup of estrogen receptor-negative tumors ( P = 0.007). In a separate set of 42 hereditary breast cancers, the majority ( 78%) of the BRCA1-associated tumors, but only one of 15 BRCA2- associated tumors was positive for CK5/ 14. In contrast to sporadic CK5/ 14- positive tumors, BRCA1- associated tumors displayed less intense CK8/ 18 staining, including some truly CK5/ 14- positive CK8/ 18- negative cases. These results suggest that CK5/ 14- positive sporadic breast cancers arise from glandularly committed progenitor cells rather than true CK8/ 18- negative basal cells.}}, author = {{Laakso, M and Loman, Niklas and Borg, Åke and Isola, J}}, issn = {{1530-0285}}, keywords = {{immunohistochemistry; HER-2; cytokeratin; BRCA2; basal phenotype; BRCA1; oncogene; progenitor cell}}, language = {{eng}}, number = {{10}}, pages = {{1321--1328}}, publisher = {{Nature Publishing Group}}, series = {{Modern Pathology}}, title = {{Cytokeratin 5/14-positive breast cancer: true basal phenotype confined to BRCA1 tumors}}, url = {{http://dx.doi.org/10.1038/modpathol.3800456}}, doi = {{10.1038/modpathol.3800456}}, volume = {{18}}, year = {{2005}}, }