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Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors

Yan, H; Guo, YH; Zhang, P; Zu, LY; Dong, XY; Chen, L; Tian, JW; Fan, Xiaolong LU ; Wang, NP and Wu, XB, et al. (2005) In Biochemical and Biophysical Research Communications 336(1). p.287-298
Abstract
Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection.... (More)
Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases. (Less)
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publication status
published
subject
keywords
endothelial growth factor, vascular, gene delivery, serotype 1, adeno-associated virus, muscle, ischemia
in
Biochemical and Biophysical Research Communications
volume
336
issue
1
pages
287 - 298
publisher
Elsevier
external identifiers
  • pmid:16129416
  • wos:000231941500042
  • scopus:24344431602
ISSN
1090-2104
DOI
10.1016/j.bbrc.2005.08.066
language
English
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yes
id
6ee7e7d8-898d-4d53-897d-abfa008a71e0 (old id 223798)
date added to LUP
2007-10-10 20:26:58
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2017-08-06 04:23:57
@article{6ee7e7d8-898d-4d53-897d-abfa008a71e0,
  abstract     = {Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases.},
  author       = {Yan, H and Guo, YH and Zhang, P and Zu, LY and Dong, XY and Chen, L and Tian, JW and Fan, Xiaolong and Wang, NP and Wu, XB and Gao, W},
  issn         = {1090-2104},
  keyword      = {endothelial growth factor,vascular,gene delivery,serotype 1,adeno-associated virus,muscle,ischemia},
  language     = {eng},
  number       = {1},
  pages        = {287--298},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2005.08.066},
  volume       = {336},
  year         = {2005},
}