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Guiding molecular motors with nano-imprinted structures

Bunk, Richard LU ; Carlberg, Patrick LU ; Mansson, A; Nicholls, IA; Omling, Pär LU ; Sundberg, M; Tagerud, S and Montelius, Lars LU (2005) In Japanese Journal of Applied Physics 44(5A). p.3337-3340
Abstract
This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution... (More)
This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution electron beam lithography. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lab-on-a-chip, myosin, actin, molecular motors, nano-imprinting, factory-on-a-chip
in
Japanese Journal of Applied Physics
volume
44
issue
5A
pages
3337 - 3340
publisher
Japan Society of Applied Physics
external identifiers
  • wos:000231874900095
  • scopus:22544477530
ISSN
0021-4922
DOI
10.1143/JJAP.44.3337
language
English
LU publication?
yes
id
5ea9b477-c078-4f6c-9abb-d351880fb85f (old id 224139)
date added to LUP
2007-08-14 08:49:16
date last changed
2017-01-01 04:37:35
@article{5ea9b477-c078-4f6c-9abb-d351880fb85f,
  abstract     = {This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution electron beam lithography.},
  author       = {Bunk, Richard and Carlberg, Patrick and Mansson, A and Nicholls, IA and Omling, Pär and Sundberg, M and Tagerud, S and Montelius, Lars},
  issn         = {0021-4922},
  keyword      = {lab-on-a-chip,myosin,actin,molecular motors,nano-imprinting,factory-on-a-chip},
  language     = {eng},
  number       = {5A},
  pages        = {3337--3340},
  publisher    = {Japan Society of Applied Physics},
  series       = {Japanese Journal of Applied Physics},
  title        = {Guiding molecular motors with nano-imprinted structures},
  url          = {http://dx.doi.org/10.1143/JJAP.44.3337},
  volume       = {44},
  year         = {2005},
}