Entamoeba gingivalis : epidemiology, genetic diversity and association with oral microbiota signatures in North Eastern Tanzania
(2021) In Journal of oral microbiology 13(1).- Abstract
Background: Entamoeba gingivalis has been associated with periodontal diseases. Baseline data from the background population, which could help delimit the role of the parasite in health and disease, remain limited. Objective: To describe epidemiological features, genetic diversity, and associations with oral microbiome signatures of E. gingivalis colonisation in Tanzanians with non-oral/non-dental diseases. Methods: DNAs from 92 oral washings from 52 participants were subject to metabarcoding of ribosomal genes. DNA sequences were identified to genus level and submitted to oral microbiota diversity analyses. Results: Sixteen (31%) of the 52 study participants were E. gingivalis-positive, with no difference in positivity rate according... (More)
Background: Entamoeba gingivalis has been associated with periodontal diseases. Baseline data from the background population, which could help delimit the role of the parasite in health and disease, remain limited. Objective: To describe epidemiological features, genetic diversity, and associations with oral microbiome signatures of E. gingivalis colonisation in Tanzanians with non-oral/non-dental diseases. Methods: DNAs from 92 oral washings from 52 participants were subject to metabarcoding of ribosomal genes. DNA sequences were identified to genus level and submitted to oral microbiota diversity analyses. Results: Sixteen (31%) of the 52 study participants were E. gingivalis-positive, with no difference in positivity rate according to gender or age. Only one subtype (ST1) was found. Individuals testing positive for E. gingivalis had higher oral microbiota alpha diversity than those testing negative (P = 0.03). Eight of the top-ten most common bacterial genera were shared between the two groups (Alloprevotella, Fusobacterium, Gemella, Haemophilus, Neisseria, Porphyromonas, Prevotella, Streptococcus, and Veillonella). Meanwhile, E. gingivalis carriers and non-carriers were more likely to have Aggregatibacter and Rothia, respectively, among the top-ten most common genera. Conclusion: About one third of the cohort carried E. gingivalis ST1, and carriers had higher oral microbiome diversity and were more predominantly colonized by Aggregatibacter.
(Less)
- author
- Stensvold, Christen Rune ; Nielsen, Michelle ; Baraka, Vito ; Lood, Rolf LU ; Fuursted, Kurt and Nielsen, Henrik Vedel
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Africa, amoeba, ngs, oral microbiota, parasite, parasitology, periodontitis, sub-saharan africa
- in
- Journal of oral microbiology
- volume
- 13
- issue
- 1
- article number
- 1924598
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:85106266474
- pmid:34104347
- ISSN
- 2000-2297
- DOI
- 10.1080/20002297.2021.1924598
- language
- English
- LU publication?
- yes
- id
- 2246e202-13c4-4d7c-b08d-2508adc4247e
- date added to LUP
- 2022-01-31 18:18:15
- date last changed
- 2024-04-20 20:51:45
@article{2246e202-13c4-4d7c-b08d-2508adc4247e,
abstract = {{<p>Background: Entamoeba gingivalis has been associated with periodontal diseases. Baseline data from the background population, which could help delimit the role of the parasite in health and disease, remain limited. Objective: To describe epidemiological features, genetic diversity, and associations with oral microbiome signatures of E. gingivalis colonisation in Tanzanians with non-oral/non-dental diseases. Methods: DNAs from 92 oral washings from 52 participants were subject to metabarcoding of ribosomal genes. DNA sequences were identified to genus level and submitted to oral microbiota diversity analyses. Results: Sixteen (31%) of the 52 study participants were E. gingivalis-positive, with no difference in positivity rate according to gender or age. Only one subtype (ST1) was found. Individuals testing positive for E. gingivalis had higher oral microbiota alpha diversity than those testing negative (P = 0.03). Eight of the top-ten most common bacterial genera were shared between the two groups (Alloprevotella, Fusobacterium, Gemella, Haemophilus, Neisseria, Porphyromonas, Prevotella, Streptococcus, and Veillonella). Meanwhile, E. gingivalis carriers and non-carriers were more likely to have Aggregatibacter and Rothia, respectively, among the top-ten most common genera. Conclusion: About one third of the cohort carried E. gingivalis ST1, and carriers had higher oral microbiome diversity and were more predominantly colonized by Aggregatibacter.</p>}},
author = {{Stensvold, Christen Rune and Nielsen, Michelle and Baraka, Vito and Lood, Rolf and Fuursted, Kurt and Nielsen, Henrik Vedel}},
issn = {{2000-2297}},
keywords = {{Africa; amoeba; ngs; oral microbiota; parasite; parasitology; periodontitis; sub-saharan africa}},
language = {{eng}},
number = {{1}},
publisher = {{Taylor & Francis}},
series = {{Journal of oral microbiology}},
title = {{Entamoeba gingivalis : epidemiology, genetic diversity and association with oral microbiota signatures in North Eastern Tanzania}},
url = {{http://dx.doi.org/10.1080/20002297.2021.1924598}},
doi = {{10.1080/20002297.2021.1924598}},
volume = {{13}},
year = {{2021}},
}