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TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells

Zhu, Chunhua; Di, Dongmei; Zhang, Xiaoying; Luo, Guanghua; Wang, Zongchun; Wei, Jiang; Shi, Yuanping; Berggren Söderlund, Maria LU ; Nilsson-Ehle, Peter LU and Xu, Ning LU (2011) In Lipids in Health and Disease 10.
Abstract
Background: Apolipoprotein M (apoM) may have potential antiatherosclerotic properties. It has been reported that apoM expression could be regulated by many intracellar and extracellar factors. In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a liver X receptor (LXR) agonist, TO901317. Materials and methods: Caco-2 cells were cultured in the presence of either TO901317, farnesoid X receptor (FXR) antagonist guggulsterone or TO901317 together with guggulsterone at different concentrations for 24 hrs. The mRNA levels of ATP-binding cassette transporter A1 (ABCA1), apoA1, apoM, liver receptor homologue-1 (LRH-1) and short heterodimer partner 1 (SHP1) were determined by real-time RT-PCR.... (More)
Background: Apolipoprotein M (apoM) may have potential antiatherosclerotic properties. It has been reported that apoM expression could be regulated by many intracellar and extracellar factors. In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a liver X receptor (LXR) agonist, TO901317. Materials and methods: Caco-2 cells were cultured in the presence of either TO901317, farnesoid X receptor (FXR) antagonist guggulsterone or TO901317 together with guggulsterone at different concentrations for 24 hrs. The mRNA levels of ATP-binding cassette transporter A1 (ABCA1), apoA1, apoM, liver receptor homologue-1 (LRH-1) and short heterodimer partner 1 (SHP1) were determined by real-time RT-PCR. Results: When Caco-2 cell cultured with TO901317 alone, the mRNA levels of ABCA1, apoA1, apoM, LRH-1 and SHP1 were significantly increased with dose-dependent manners (p < 0.05), whereas when the cells cultured with guggulsterone alone, the mRNA levels of apoM, SHP1 and LRH-1 (p < 0.05) were strongly inhibited. Moreover, guggulsterone could abolish the TO901317 enhanced mRNA levels of apoA1 apoM, SHP1 and LRH-1. Conclusion: The present study demonstrated that LXR agonist TO901317 induced apoM expression in Caco-2 cells might be mediated via the LXR/FXR pathway. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Liver X Receptor, Farnesoid X Receptor, Caco-2 cell line, Apolipoprotein M
in
Lipids in Health and Disease
volume
10
publisher
BioMed Central
external identifiers
  • wos:000297109000001
  • scopus:80155147896
ISSN
1476-511X
DOI
10.1186/1476-511X-10-199
language
English
LU publication?
yes
id
5b809690-c8bd-43e4-a763-c01c39e54612 (old id 2252672)
date added to LUP
2012-01-02 07:56:55
date last changed
2017-07-23 04:15:20
@article{5b809690-c8bd-43e4-a763-c01c39e54612,
  abstract     = {Background: Apolipoprotein M (apoM) may have potential antiatherosclerotic properties. It has been reported that apoM expression could be regulated by many intracellar and extracellar factors. In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a liver X receptor (LXR) agonist, TO901317. Materials and methods: Caco-2 cells were cultured in the presence of either TO901317, farnesoid X receptor (FXR) antagonist guggulsterone or TO901317 together with guggulsterone at different concentrations for 24 hrs. The mRNA levels of ATP-binding cassette transporter A1 (ABCA1), apoA1, apoM, liver receptor homologue-1 (LRH-1) and short heterodimer partner 1 (SHP1) were determined by real-time RT-PCR. Results: When Caco-2 cell cultured with TO901317 alone, the mRNA levels of ABCA1, apoA1, apoM, LRH-1 and SHP1 were significantly increased with dose-dependent manners (p &lt; 0.05), whereas when the cells cultured with guggulsterone alone, the mRNA levels of apoM, SHP1 and LRH-1 (p &lt; 0.05) were strongly inhibited. Moreover, guggulsterone could abolish the TO901317 enhanced mRNA levels of apoA1 apoM, SHP1 and LRH-1. Conclusion: The present study demonstrated that LXR agonist TO901317 induced apoM expression in Caco-2 cells might be mediated via the LXR/FXR pathway.},
  author       = {Zhu, Chunhua and Di, Dongmei and Zhang, Xiaoying and Luo, Guanghua and Wang, Zongchun and Wei, Jiang and Shi, Yuanping and Berggren Söderlund, Maria and Nilsson-Ehle, Peter and Xu, Ning},
  issn         = {1476-511X},
  keyword      = {Liver X Receptor,Farnesoid X Receptor,Caco-2 cell line,Apolipoprotein M},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {Lipids in Health and Disease},
  title        = {TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells},
  url          = {http://dx.doi.org/10.1186/1476-511X-10-199},
  volume       = {10},
  year         = {2011},
}