Advanced

Preservation effects on stable isotope ratios and consequences for the reconstruction of energetic pathways

Xu, Jun LU ; Yang, Qiang; Zhang, Meng; Zhang, Min; Xie, Ping and Hansson, Lars-Anders LU (2011) In Aquatic Ecology 45(4). p.483-492
Abstract
Stable isotope analysis provides a powerful tool for describing the energetic pathways in a variety of ecosystems. However, isotope ratios of animal tissues can be altered by preservation methods, potentially leading to biased estimates of energy pathways when they are not taken into account. Here, we investigated the direct preservation effects of formalin, ethanol, NaCl, and drying on the delta C-13 and delta N-15 of fish muscle tissues, as well as the ultimate effects on the reconstruction of the energy pathways. All preservation methods, except drying, had significant impacts on delta C-13 and delta N-15 values. The effects of preservation appear to be highly taxa-specific and no significant time-dependent variations in nearly 2-year... (More)
Stable isotope analysis provides a powerful tool for describing the energetic pathways in a variety of ecosystems. However, isotope ratios of animal tissues can be altered by preservation methods, potentially leading to biased estimates of energy pathways when they are not taken into account. Here, we investigated the direct preservation effects of formalin, ethanol, NaCl, and drying on the delta C-13 and delta N-15 of fish muscle tissues, as well as the ultimate effects on the reconstruction of the energy pathways. All preservation methods, except drying, had significant impacts on delta C-13 and delta N-15 values. The effects of preservation appear to be highly taxa-specific and no significant time-dependent variations in nearly 2-year duration of preservation. delta C-13 and delta N-15 values were generally changed dramatically within the early stage of the preservation process and became stable over a relatively long-term preservation. Using an isotopic balance mixing model, the isotope-based food web reconstruction reveals that, without preservation correction, the importance of the pelagic energetic pathways for the fishes could be misestimated, except for the drying preservation. These results highlight that preservation can bias the interpretation of food web reconstruction results. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Carbon and nitrogen, Preservation, Food web reconstruction, Trophic, relationships, Energy flows
in
Aquatic Ecology
volume
45
issue
4
pages
483 - 492
publisher
Springer
external identifiers
  • wos:000296632800004
  • scopus:80355144515
ISSN
1386-2588
DOI
10.1007/s10452-011-9369-5
language
English
LU publication?
yes
id
6f70a298-b17a-41d4-a6d4-cb34d5c68151 (old id 2254566)
date added to LUP
2011-12-20 08:09:14
date last changed
2017-09-17 06:54:28
@article{6f70a298-b17a-41d4-a6d4-cb34d5c68151,
  abstract     = {Stable isotope analysis provides a powerful tool for describing the energetic pathways in a variety of ecosystems. However, isotope ratios of animal tissues can be altered by preservation methods, potentially leading to biased estimates of energy pathways when they are not taken into account. Here, we investigated the direct preservation effects of formalin, ethanol, NaCl, and drying on the delta C-13 and delta N-15 of fish muscle tissues, as well as the ultimate effects on the reconstruction of the energy pathways. All preservation methods, except drying, had significant impacts on delta C-13 and delta N-15 values. The effects of preservation appear to be highly taxa-specific and no significant time-dependent variations in nearly 2-year duration of preservation. delta C-13 and delta N-15 values were generally changed dramatically within the early stage of the preservation process and became stable over a relatively long-term preservation. Using an isotopic balance mixing model, the isotope-based food web reconstruction reveals that, without preservation correction, the importance of the pelagic energetic pathways for the fishes could be misestimated, except for the drying preservation. These results highlight that preservation can bias the interpretation of food web reconstruction results.},
  author       = {Xu, Jun and Yang, Qiang and Zhang, Meng and Zhang, Min and Xie, Ping and Hansson, Lars-Anders},
  issn         = {1386-2588},
  keyword      = {Carbon and nitrogen,Preservation,Food web reconstruction,Trophic,relationships,Energy flows},
  language     = {eng},
  number       = {4},
  pages        = {483--492},
  publisher    = {Springer},
  series       = {Aquatic Ecology},
  title        = {Preservation effects on stable isotope ratios and consequences for the reconstruction of energetic pathways},
  url          = {http://dx.doi.org/10.1007/s10452-011-9369-5},
  volume       = {45},
  year         = {2011},
}