Advanced

Fluorescence imaging of frit effects in capillary separations

Behnke, B; Johansson, Jonas LU ; Bayer, E and Nilsson, S (2000) In Electrophoresis 21(15). p.3102-3108
Abstract
The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase... (More)
The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase packing material were studied as well as at the transition from stationary phase to the outlet frit and the open tubular area. Striking differences in signal intensity and separation efficiency were revealed depending on where on the column detection was performed. Furthermore, adsorption of the analytes on the frits was observed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electrophoresis
volume
21
issue
15
pages
3102 - 3108
publisher
John Wiley & Sons
external identifiers
  • scopus:0033804520
ISSN
0173-0835
DOI
10.1002/1522-2683(20000901)
language
English
LU publication?
yes
id
c227da87-05be-4000-ba6f-13e9163b772c (old id 2257426)
date added to LUP
2012-02-23 23:34:03
date last changed
2017-01-01 07:40:31
@article{c227da87-05be-4000-ba6f-13e9163b772c,
  abstract     = {The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase packing material were studied as well as at the transition from stationary phase to the outlet frit and the open tubular area. Striking differences in signal intensity and separation efficiency were revealed depending on where on the column detection was performed. Furthermore, adsorption of the analytes on the frits was observed.},
  author       = {Behnke, B and Johansson, Jonas and Bayer, E and Nilsson, S},
  issn         = {0173-0835},
  language     = {eng},
  number       = {15},
  pages        = {3102--3108},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Fluorescence imaging of frit effects in capillary separations},
  url          = {http://dx.doi.org/10.1002/1522-2683(20000901)},
  volume       = {21},
  year         = {2000},
}