Laser-induced fluorescence studies of normal and malignant tumour tissue of rat following intravenous injection of delta-amino levulinic acid
(1997) In Lasers in Surgery and Medicine 20(3). p.272-279- Abstract
- Background and Objective: Laser-induced fluorescence was studied in normal and tumour tissue of rat after intravenous injection of delta-amino levulinic acid (ALA). The aim of the study was to investigate the protoporphyrin IX accumulation in different tissue types in rat after systemically administered ALA. Study Design/Material and Methods: A malignant rat tumour and normal tissue from 13 different organs were investigated in eight rats. The rats were injected with two different ALA doses, 30 and 90 mg/kg b.w., and the investigations were performed at 10, 30, and 240 min after the injection. The fluorescence was recorded utilising an optical fibre based fluorosensor at 405 nm excitation. Results: Fluorescence spectra were recorded in the... (More)
- Background and Objective: Laser-induced fluorescence was studied in normal and tumour tissue of rat after intravenous injection of delta-amino levulinic acid (ALA). The aim of the study was to investigate the protoporphyrin IX accumulation in different tissue types in rat after systemically administered ALA. Study Design/Material and Methods: A malignant rat tumour and normal tissue from 13 different organs were investigated in eight rats. The rats were injected with two different ALA doses, 30 and 90 mg/kg b.w., and the investigations were performed at 10, 30, and 240 min after the injection. The fluorescence was recorded utilising an optical fibre based fluorosensor at 405 nm excitation. Results: Fluorescence spectra were recorded in the 400-750 nm wavelength region including the dual-peaked PpIX fluorescence at about 635 and 705 nm, and the tissue autofluorescence peaking at about 500 nm, The maximum tumour build-up of PpIX was achieved already in less than 1 hr after ALA injection. The fluorescence demarcation between tumour and surrounding tissue was a factor of 7-8:1 after 30 min and decreased for longer retention times. The accumulation in 13 different organs was investigated and a particularly high PpIX build-up was found in stomach and intestine. Conclusions: Fluorescence detection following i.v. injection of ALA provides attractive diagnostics for the experimental tumour used, indicating clinical usefulness. (C) 1997 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2258488
- author
- Johansson, Jonas LU ; Berg, R ; Svanberg, Katarina LU and Svanberg, Sune LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lasers in Surgery and Medicine
- volume
- 20
- issue
- 3
- pages
- 272 - 279
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0030997011
- ISSN
- 0196-8092
- DOI
- 10.1002/(SICI)1096-9101(1997)20:3<272::AID-LSM6>3.0.CO;2-N
- language
- English
- LU publication?
- yes
- id
- 0287de27-c235-4034-acdf-7e64ca82b3f4 (old id 2258488)
- date added to LUP
- 2016-04-04 09:18:02
- date last changed
- 2022-03-08 00:04:36
@article{0287de27-c235-4034-acdf-7e64ca82b3f4,
abstract = {{Background and Objective: Laser-induced fluorescence was studied in normal and tumour tissue of rat after intravenous injection of delta-amino levulinic acid (ALA). The aim of the study was to investigate the protoporphyrin IX accumulation in different tissue types in rat after systemically administered ALA. Study Design/Material and Methods: A malignant rat tumour and normal tissue from 13 different organs were investigated in eight rats. The rats were injected with two different ALA doses, 30 and 90 mg/kg b.w., and the investigations were performed at 10, 30, and 240 min after the injection. The fluorescence was recorded utilising an optical fibre based fluorosensor at 405 nm excitation. Results: Fluorescence spectra were recorded in the 400-750 nm wavelength region including the dual-peaked PpIX fluorescence at about 635 and 705 nm, and the tissue autofluorescence peaking at about 500 nm, The maximum tumour build-up of PpIX was achieved already in less than 1 hr after ALA injection. The fluorescence demarcation between tumour and surrounding tissue was a factor of 7-8:1 after 30 min and decreased for longer retention times. The accumulation in 13 different organs was investigated and a particularly high PpIX build-up was found in stomach and intestine. Conclusions: Fluorescence detection following i.v. injection of ALA provides attractive diagnostics for the experimental tumour used, indicating clinical usefulness. (C) 1997 Wiley-Liss, Inc.}},
author = {{Johansson, Jonas and Berg, R and Svanberg, Katarina and Svanberg, Sune}},
issn = {{0196-8092}},
language = {{eng}},
number = {{3}},
pages = {{272--279}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Lasers in Surgery and Medicine}},
title = {{Laser-induced fluorescence studies of normal and malignant tumour tissue of rat following intravenous injection of delta-amino levulinic acid}},
url = {{https://lup.lub.lu.se/search/files/5286570/2297311.pdf}},
doi = {{10.1002/(SICI)1096-9101(1997)20:3<272::AID-LSM6>3.0.CO;2-N}},
volume = {{20}},
year = {{1997}},
}