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Fluorescence imaging of light absorption for axial-beam geometry in capillary electrophoresis

Johansson, Jonas LU ; Johansson, T and Nilsson, S (1998) In Electrophoresis 19(12). p.2233-2238
Abstract
A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along the capillary. As the UV light propagates through the sample peaks, an additional loss in intensity will be observed. In order to view the absorption profile along the capillary, a background fluorophore is added to the buffer. A charge-coupled device (CCD) detector and imaging optics are placed beside the capillary to view the capillary in a direction perpendicular to the capillary. Signal integration is... (More)
A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along the capillary. As the UV light propagates through the sample peaks, an additional loss in intensity will be observed. In order to view the absorption profile along the capillary, a background fluorophore is added to the buffer. A charge-coupled device (CCD) detector and imaging optics are placed beside the capillary to view the capillary in a direction perpendicular to the capillary. Signal integration is employed for consecutive exposures as well as for neighboring detector pixels in order to increase the signal-to-noise ratio. Measurements for stilbene 3 with sulforhodamine B as a background fluorophore are presented. The characteristics of the detection method and potential improvements are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electrophoresis
volume
19
issue
12
pages
2233 - 2238
publisher
John Wiley & Sons
external identifiers
  • scopus:0031691149
ISSN
0173-0835
DOI
10.1002/elps.1150191231
language
English
LU publication?
yes
id
bbbe329d-5b9c-4fc2-a487-6fdd9a0270f2 (old id 2258496)
date added to LUP
2012-02-20 20:56:57
date last changed
2017-01-01 07:32:25
@article{bbbe329d-5b9c-4fc2-a487-6fdd9a0270f2,
  abstract     = {A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along the capillary. As the UV light propagates through the sample peaks, an additional loss in intensity will be observed. In order to view the absorption profile along the capillary, a background fluorophore is added to the buffer. A charge-coupled device (CCD) detector and imaging optics are placed beside the capillary to view the capillary in a direction perpendicular to the capillary. Signal integration is employed for consecutive exposures as well as for neighboring detector pixels in order to increase the signal-to-noise ratio. Measurements for stilbene 3 with sulforhodamine B as a background fluorophore are presented. The characteristics of the detection method and potential improvements are discussed.},
  author       = {Johansson, Jonas and Johansson, T and Nilsson, S},
  issn         = {0173-0835},
  language     = {eng},
  number       = {12},
  pages        = {2233--2238},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Fluorescence imaging of light absorption for axial-beam geometry in capillary electrophoresis},
  url          = {http://dx.doi.org/10.1002/elps.1150191231},
  volume       = {19},
  year         = {1998},
}